BRENDA - Enzyme Database

A multilocus system for studying tissue and subcellular specialization. The three NADP-dependent isocitrate dehydrogenase isozymes of the fish Fundulus heteroclitus

Gonzalez-Villasenor, L.I.; Powers, D.A.; J. Biol. Chem. 260, 9106-9113 (1985)

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
cytoplasm
-
Fundulus heteroclitus
5737
-
1.1.1.42
mitochondrion
-
Fundulus heteroclitus
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
absolute requirement for divalent cations
Fundulus heteroclitus
1.1.1.42
Mn2+
absolute requirement for divalent cations
Fundulus heteroclitus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
90000
-
gel filtration, 3 isoenzymes
Fundulus heteroclitus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Fundulus heteroclitus
-
2-oxoglutarate + NADPH + CO2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Fundulus heteroclitus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.42
-
Fundulus heteroclitus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
brain
-
Fundulus heteroclitus
-
1.1.1.42
intestine
-
Fundulus heteroclitus
-
1.1.1.42
liver
-
Fundulus heteroclitus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286792
Fundulus heteroclitus
2-oxoglutarate + NADPH + CO2
-
-
-
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.42
35
-
isoenzyme C, half-life 20 min
Fundulus heteroclitus
1.1.1.42
49
-
isoenzyme A, half-life 20 min
Fundulus heteroclitus
1.1.1.42
52
-
isoenzyme C, half-life 20 min, presence of isocitrate
Fundulus heteroclitus
1.1.1.42
54
-
isoenzyme B, T50-value
Fundulus heteroclitus
1.1.1.42
58
-
isoenzyme A, T50-value, presence of isocitrate
Fundulus heteroclitus
1.1.1.42
69
-
isoenzyme B, T50-value, presence of isocitrate
Fundulus heteroclitus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
cytoplasm
-
Fundulus heteroclitus
5737
-
1.1.1.42
mitochondrion
-
Fundulus heteroclitus
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
absolute requirement for divalent cations
Fundulus heteroclitus
1.1.1.42
Mn2+
absolute requirement for divalent cations
Fundulus heteroclitus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
90000
-
gel filtration, 3 isoenzymes
Fundulus heteroclitus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Fundulus heteroclitus
-
2-oxoglutarate + NADPH + CO2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
-
Fundulus heteroclitus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
brain
-
Fundulus heteroclitus
-
1.1.1.42
intestine
-
Fundulus heteroclitus
-
1.1.1.42
liver
-
Fundulus heteroclitus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286792
Fundulus heteroclitus
2-oxoglutarate + NADPH + CO2
-
-
-
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.42
35
-
isoenzyme C, half-life 20 min
Fundulus heteroclitus
1.1.1.42
49
-
isoenzyme A, half-life 20 min
Fundulus heteroclitus
1.1.1.42
52
-
isoenzyme C, half-life 20 min, presence of isocitrate
Fundulus heteroclitus
1.1.1.42
54
-
isoenzyme B, T50-value
Fundulus heteroclitus
1.1.1.42
58
-
isoenzyme A, T50-value, presence of isocitrate
Fundulus heteroclitus
1.1.1.42
69
-
isoenzyme B, T50-value, presence of isocitrate
Fundulus heteroclitus