BRENDA - Enzyme Database

Isolation and characterization of NADP+-specific isocitrate dehydrogenase from the pupa of Bombyx mori

Miake, F.; Torikata, T.; Koga, K.; Hayashi, K.; J. Biochem. 82, 449-454 (1977)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
2-oxoglutarate
substrate inhibition
Bombyx mori
1.1.1.42
adenosine-3',5'-cyclic monophosphate
-
Bombyx mori
1.1.1.42
Ag+
decarboxylation of isocitrate and oxalosuccinate
Bombyx mori
1.1.1.42
citrate
-
Bombyx mori
1.1.1.42
Maleate
-
Bombyx mori
1.1.1.42
monoiodoacetate
-
Bombyx mori
1.1.1.42
oxaloacetate
-
Bombyx mori
1.1.1.42
p-chloromercuribenzoate
-
Bombyx mori
1.1.1.42
Zn2+
-
Bombyx mori
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Co2+
absolute requirement for divalent cations
Bombyx mori
1.1.1.42
Mg2+
absolute requirement for divalent cations
Bombyx mori
1.1.1.42
Mn2+
absolute requirement for divalent cations
Bombyx mori
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
86000
-
gel filtration
Bombyx mori
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Bombyx mori
-
2-oxoglutarate + NADPH + CO2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Bombyx mori
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.42
-
Bombyx mori
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
pupa
-
Bombyx mori
-
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.42
-20°C, 6 months, 10% loss of activity
Bombyx mori
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286784
Bombyx mori
2-oxoglutarate + NADPH + CO2
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.42
23
-
-
Bombyx mori
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.42
45
-
-
Bombyx mori
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
6.6
-
2-oxoglutarate + CO2 + NADPH
Bombyx mori
1.1.1.42
7.8
-
isocitrate + NADP+
Bombyx mori
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
2-oxoglutarate
substrate inhibition
Bombyx mori
1.1.1.42
adenosine-3',5'-cyclic monophosphate
-
Bombyx mori
1.1.1.42
Ag+
decarboxylation of isocitrate and oxalosuccinate
Bombyx mori
1.1.1.42
citrate
-
Bombyx mori
1.1.1.42
Maleate
-
Bombyx mori
1.1.1.42
monoiodoacetate
-
Bombyx mori
1.1.1.42
oxaloacetate
-
Bombyx mori
1.1.1.42
p-chloromercuribenzoate
-
Bombyx mori
1.1.1.42
Zn2+
-
Bombyx mori
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Co2+
absolute requirement for divalent cations
Bombyx mori
1.1.1.42
Mg2+
absolute requirement for divalent cations
Bombyx mori
1.1.1.42
Mn2+
absolute requirement for divalent cations
Bombyx mori
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
86000
-
gel filtration
Bombyx mori
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Bombyx mori
-
2-oxoglutarate + NADPH + CO2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
-
Bombyx mori
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
pupa
-
Bombyx mori
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.42
-20°C, 6 months, 10% loss of activity
Bombyx mori
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286784
Bombyx mori
2-oxoglutarate + NADPH + CO2
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.42
23
-
-
Bombyx mori
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.42
45
-
-
Bombyx mori
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
6.6
-
2-oxoglutarate + CO2 + NADPH
Bombyx mori
1.1.1.42
7.8
-
isocitrate + NADP+
Bombyx mori