BRENDA - Enzyme Database

Purification, properties, and kinetic observations on the isoenzymes of NADP isocitrate dehydrogenase of maize

Curry, R.A.; Ting, I.P.; Arch. Biochem. Biophys. 176, 501-509 (1976)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.1.1.42
instable in solutions of low ionic strength, stabilization by ammonium sulfate
Sus scrofa
1.1.1.42
instable in solutions of low ionic strength, stabilization by ammonium sulfate
Zea mays
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.0017
-
D,L-isocitrate
isozyme 2
Zea mays
1.1.1.42
0.003
-
NADP+
isozyme 2
Zea mays
1.1.1.42
0.0031
-
D,L-isocitrate
isozyme 1
Zea mays
1.1.1.42
0.0063
-
NADP+
isozyme 1
Zea mays
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
mitochondrion
-
Azotobacter sp.
5739
-
1.1.1.42
mitochondrion
-
Geobacillus stearothermophilus
5739
-
1.1.1.42
mitochondrion
-
Rhodobacter sphaeroides
5739
-
1.1.1.42
mitochondrion
-
Zea mays
5739
-
1.1.1.42
mitochondrion
-
Sus scrofa
5739
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
68000
-
gel filtration
Sus scrofa
1.1.1.42
81000
83000
gel filtration
Zea mays
1.1.1.42
105000
-
gel filtration
Rhodobacter sphaeroides
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Sus scrofa
-
2-oxoglutarate + NADPH + CO2
-
-
-
1.1.1.42
isocitrate + NADP+
Geobacillus stearothermophilus
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Rhodobacter sphaeroides
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Zea mays
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Azotobacter sp.
-
2-oxoglutarate + NADPH + CO2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Azotobacter sp.
-
-
-
1.1.1.42
Geobacillus stearothermophilus
-
-
-
1.1.1.42
Rhodobacter sphaeroides
-
i.e. Rhodobacter sphaeroides
-
1.1.1.42
Sus scrofa
-
pig
-
1.1.1.42
Zea mays
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.42
-
Zea mays
Renatured (Commentary)
EC Number
Commentary
Organism
1.1.1.42
overnight dialysis against guanidine-HCl, GDP buffer
Azotobacter sp.
1.1.1.42
overnight dialysis against guanidine-HCl, GDP buffer
Geobacillus stearothermophilus
1.1.1.42
overnight dialysis against guanidine-HCl, GDP buffer
Rhodobacter sphaeroides
1.1.1.42
overnight dialysis against guanidine-HCl, GDP buffer
Sus scrofa
1.1.1.42
overnight dialysis against guanidine-HCl, GDP buffer
Zea mays
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
heart
-
Sus scrofa
-
1.1.1.42
seed
-
Zea mays
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.42
33.5
-
-
Zea mays
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286782
Sus scrofa
2-oxoglutarate + NADPH + CO2
-
-
-
-
1.1.1.42
isocitrate + NADP+
-
286782
Geobacillus stearothermophilus
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286782
Rhodobacter sphaeroides
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286782
Zea mays
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286782
Azotobacter sp.
2-oxoglutarate + NADPH + CO2
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7.4
-
-
Zea mays
General Stability (protein specific)
EC Number
General Stability
Organism
1.1.1.42
instable in solutions of low ionic strength, stabilization by ammonium sulfate
Sus scrofa
1.1.1.42
instable in solutions of low ionic strength, stabilization by ammonium sulfate
Zea mays
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.0017
-
D,L-isocitrate
isozyme 2
Zea mays
1.1.1.42
0.003
-
NADP+
isozyme 2
Zea mays
1.1.1.42
0.0031
-
D,L-isocitrate
isozyme 1
Zea mays
1.1.1.42
0.0063
-
NADP+
isozyme 1
Zea mays
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
mitochondrion
-
Azotobacter sp.
5739
-
1.1.1.42
mitochondrion
-
Geobacillus stearothermophilus
5739
-
1.1.1.42
mitochondrion
-
Rhodobacter sphaeroides
5739
-
1.1.1.42
mitochondrion
-
Zea mays
5739
-
1.1.1.42
mitochondrion
-
Sus scrofa
5739
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
68000
-
gel filtration
Sus scrofa
1.1.1.42
81000
83000
gel filtration
Zea mays
1.1.1.42
105000
-
gel filtration
Rhodobacter sphaeroides
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Sus scrofa
-
2-oxoglutarate + NADPH + CO2
-
-
-
1.1.1.42
isocitrate + NADP+
Geobacillus stearothermophilus
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Rhodobacter sphaeroides
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Zea mays
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Azotobacter sp.
-
2-oxoglutarate + NADPH + CO2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
-
Zea mays
Renatured (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
overnight dialysis against guanidine-HCl, GDP buffer
Azotobacter sp.
1.1.1.42
overnight dialysis against guanidine-HCl, GDP buffer
Geobacillus stearothermophilus
1.1.1.42
overnight dialysis against guanidine-HCl, GDP buffer
Rhodobacter sphaeroides
1.1.1.42
overnight dialysis against guanidine-HCl, GDP buffer
Sus scrofa
1.1.1.42
overnight dialysis against guanidine-HCl, GDP buffer
Zea mays
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
heart
-
Sus scrofa
-
1.1.1.42
seed
-
Zea mays
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.42
33.5
-
-
Zea mays
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286782
Sus scrofa
2-oxoglutarate + NADPH + CO2
-
-
-
-
1.1.1.42
isocitrate + NADP+
-
286782
Geobacillus stearothermophilus
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286782
Rhodobacter sphaeroides
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286782
Zea mays
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286782
Azotobacter sp.
2-oxoglutarate + NADPH + CO2
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7.4
-
-
Zea mays