BRENDA - Enzyme Database

Purification and characterization of NAD-isocitrate dehydrogenase from Chlamydomonas reinhardtii

Martinez-Rivas, J.M.; Vega, J.M.; Plant Physiol. 118, 249-255 (1998)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.41
ADP
10 mM
Chlamydomonas reinhardtii
1.1.1.41
ATP
10 mM
Chlamydomonas reinhardtii
1.1.1.41
citrate
10 mM
Chlamydomonas reinhardtii
1.1.1.41
Cl-
100 mM
Chlamydomonas reinhardtii
1.1.1.41
I-
100 mM
Chlamydomonas reinhardtii
1.1.1.41
NADH
competitive inhibitor
Chlamydomonas reinhardtii
1.1.1.41
NADPH
noncompetitive inhibitor
Chlamydomonas reinhardtii
1.1.1.41
NO3-
100 mM
Chlamydomonas reinhardtii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.41
45000
-
8 * 45000, SDS-PAGE
Chlamydomonas reinhardtii
1.1.1.41
320000
-
gel filtration
Chlamydomonas reinhardtii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.41
isocitrate + NAD+
Chlamydomonas reinhardtii
-
2-oxoglutarate + CO2 + NADH
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.41
Chlamydomonas reinhardtii
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.41
-
Chlamydomonas reinhardtii
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.41
16.6
-
-
Chlamydomonas reinhardtii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
-
286773
Chlamydomonas reinhardtii
2-oxoglutarate + CO2 + NADH
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.41
octamer
8 * 45000, SDS-PAGE
Chlamydomonas reinhardtii
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.41
42
-
10 min, 50% loss in activity
Chlamydomonas reinhardtii
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.41
NAD+
-
Chlamydomonas reinhardtii
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.41
NAD+
-
Chlamydomonas reinhardtii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.41
ADP
10 mM
Chlamydomonas reinhardtii
1.1.1.41
ATP
10 mM
Chlamydomonas reinhardtii
1.1.1.41
citrate
10 mM
Chlamydomonas reinhardtii
1.1.1.41
Cl-
100 mM
Chlamydomonas reinhardtii
1.1.1.41
I-
100 mM
Chlamydomonas reinhardtii
1.1.1.41
NADH
competitive inhibitor
Chlamydomonas reinhardtii
1.1.1.41
NADPH
noncompetitive inhibitor
Chlamydomonas reinhardtii
1.1.1.41
NO3-
100 mM
Chlamydomonas reinhardtii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.41
45000
-
8 * 45000, SDS-PAGE
Chlamydomonas reinhardtii
1.1.1.41
320000
-
gel filtration
Chlamydomonas reinhardtii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.41
isocitrate + NAD+
Chlamydomonas reinhardtii
-
2-oxoglutarate + CO2 + NADH
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.41
-
Chlamydomonas reinhardtii
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.41
16.6
-
-
Chlamydomonas reinhardtii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
-
286773
Chlamydomonas reinhardtii
2-oxoglutarate + CO2 + NADH
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.41
octamer
8 * 45000, SDS-PAGE
Chlamydomonas reinhardtii
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.41
42
-
10 min, 50% loss in activity
Chlamydomonas reinhardtii