BRENDA - Enzyme Database

Isocitrate dehydrogenase

Plaut, G.W.E.; The Enzymes, 2nd Ed. (Boyer, P. D. , Lardy, H. , Myrb├Ąck, K. , eds. ) 7, 105-126 (1963)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.41
AMP
-
Saccharomyces cerevisiae
General Stability
EC Number
General Stability
Organism
1.1.1.42
instable in solutions of low ionic strength, stabilization by ammonium sulfate
Sus scrofa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.41
ATP
-
Bos taurus
1.1.1.41
CN-
-
Bos taurus
1.1.1.41
CN-
-
Pisum sativum
1.1.1.41
CN-
-
Saccharomyces cerevisiae
1.1.1.41
molybdate
-
Bos taurus
1.1.1.41
molybdate
-
Saccharomyces cerevisiae
1.1.1.41
N3-
-
Bos taurus
1.1.1.41
N3-
-
Saccharomyces cerevisiae
1.1.1.41
p-hydroxymercuribenzoate
-
Bos taurus
1.1.1.41
thiocyanate
-
Bos taurus
1.1.1.42
Ag+
decarboxylation of isocitrate and oxalosuccinate
Sus scrofa
1.1.1.42
Cu2+
-
Sus scrofa
1.1.1.42
Diphenylchloroarsine
-
Sus scrofa
1.1.1.42
isocitrate
competitive inhibition of decarboxylation of oxalosuccinate
Sus scrofa
1.1.1.42
p-chloromercuribenzoate
-
Sus scrofa
1.1.1.42
p-hydroxymercuribenzoate
decarboxylation of isocitrate and oxalosuccinate
Sus scrofa
1.1.1.42
Phenarsazines
-
Sus scrofa
1.1.1.42
Phenylmercuric nitrate
-
Sus scrofa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.41
0.11
-
D,L-isocitrate
-
Saccharomyces cerevisiae
1.1.1.41
0.45
-
D,L-isocitrate
-
Bos taurus
1.1.1.42
0.0026
-
D,L-isocitrate
-
Sus scrofa
1.1.1.42
0.0092
-
NADPH
-
Sus scrofa
1.1.1.42
0.13
-
2-oxoglutarate
-
Sus scrofa
1.1.1.42
0.56
-
oxalosuccinate
reductase
Sus scrofa
1.1.1.42
1.2
-
oxalosuccinate
decarboxylase
Sus scrofa
1.1.1.42
25
26
oxalosuccinate
decarboxylase
Sus scrofa
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.41
mitochondrion
-
Aspergillus niger
5739
-
1.1.1.41
mitochondrion
-
Cavia porcellus
5739
-
1.1.1.41
mitochondrion
-
Columba livia
5739
-
1.1.1.41
mitochondrion
-
Bos taurus
5739
-
1.1.1.41
mitochondrion
-
Pisum sativum
5739
-
1.1.1.41
mitochondrion
-
Saccharomyces cerevisiae
5739
-
1.1.1.41
mitochondrion
-
Homo sapiens
5739
-
1.1.1.41
mitochondrion
-
Rattus norvegicus
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.41
Mg2+
-
Aspergillus niger
1.1.1.41
Mg2+
-
Bos taurus
1.1.1.41
Mg2+
-
Pisum sativum
1.1.1.41
Mg2+
-
Saccharomyces cerevisiae
1.1.1.41
Mn2+
requires divalent cations
Bos taurus
1.1.1.41
Mn2+
requires divalent cations
Saccharomyces cerevisiae
1.1.1.41
Mn2+
requires divalent cations
Pisum sativum
1.1.1.42
Mg2+
absolute requirement for divalent cations
Aspergillus niger
1.1.1.42
Mg2+
absolute requirement for divalent cations
Sus scrofa
1.1.1.42
Mn2+
absolute requirement for divalent cations
Aspergillus niger
1.1.1.42
Mn2+
absolute requirement for divalent cations
Sus scrofa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
61000
64000
ultracentrifugation
Sus scrofa
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.41
isocitrate + NAD+
Cavia porcellus
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Homo sapiens
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Rattus norvegicus
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Saccharomyces cerevisiae
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Bos taurus
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Aspergillus niger
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Pisum sativum
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Columba livia
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.42
isocitrate + NADP+
Sus scrofa
-
2-oxoglutarate + NADPH + CO2
-
-
r
1.1.1.42
isocitrate + NADP+
Saccharomyces cerevisiae
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Aspergillus niger
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Mycobacterium tuberculosis
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Trypanosoma cruzi
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Citrobacter freundii
-
2-oxoglutarate + NADPH + CO2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.41
Aspergillus niger
-
-
-
1.1.1.41
Bos taurus
-
-
-
1.1.1.41
Cavia porcellus
-
-
-
1.1.1.41
Columba livia
-
pigeon
-
1.1.1.41
Homo sapiens
-
-
-
1.1.1.41
Pisum sativum
-
pea
-
1.1.1.41
Rattus norvegicus
-
-
-
1.1.1.41
Saccharomyces cerevisiae
-
-
-
1.1.1.42
Aspergillus niger
-
-
-
1.1.1.42
Citrobacter freundii
-
-
-
1.1.1.42
Mycobacterium tuberculosis
-
-
-
1.1.1.42
Saccharomyces cerevisiae
-
-
-
1.1.1.42
Sus scrofa
-
pig
-
1.1.1.42
Trypanosoma cruzi
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.41
heart
-
Cavia porcellus
-
1.1.1.41
heart
-
Bos taurus
-
1.1.1.41
heart
-
Homo sapiens
-
1.1.1.41
heart
-
Rattus norvegicus
-
1.1.1.41
muscle
breast muscle
Columba livia
-
1.1.1.41
muscle
-
Homo sapiens
-
1.1.1.41
placenta
-
Homo sapiens
-
1.1.1.42
heart
-
Sus scrofa
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
-
286749
Cavia porcellus
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
286749
Homo sapiens
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
286749
Rattus norvegicus
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
286749
Saccharomyces cerevisiae
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
286749
Bos taurus
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
286749
Aspergillus niger
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
286749
Pisum sativum
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
286749
Columba livia
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286749
Sus scrofa
2-oxoglutarate + NADPH + CO2
-
-
-
r
1.1.1.42
isocitrate + NADP+
-
286749
Saccharomyces cerevisiae
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286749
Aspergillus niger
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286749
Mycobacterium tuberculosis
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286749
Trypanosoma cruzi
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286749
Citrobacter freundii
2-oxoglutarate + NADPH + CO2
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.42
58.3
-
isocitrate
oxidative decarboxylation
Sus scrofa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.41
6.5
-
-
Bos taurus
1.1.1.41
7.5
-
-
Pisum sativum
1.1.1.41
7.5
-
-
Saccharomyces cerevisiae
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.41
6.5
8.5
-
Pisum sativum
1.1.1.41
6.5
8.5
-
Saccharomyces cerevisiae
1.1.1.42
5.6
8.5
-
Sus scrofa
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.41
NAD+
-
Aspergillus niger
1.1.1.41
NAD+
-
Cavia porcellus
1.1.1.41
NAD+
-
Columba livia
1.1.1.41
NAD+
-
Bos taurus
1.1.1.41
NAD+
-
Pisum sativum
1.1.1.41
NAD+
-
Saccharomyces cerevisiae
1.1.1.41
NAD+
-
Homo sapiens
1.1.1.41
NAD+
-
Rattus norvegicus
1.1.1.42
NADP+
natural coenzyme
Aspergillus niger
1.1.1.42
NADP+
natural coenzyme
Citrobacter freundii
1.1.1.42
NADP+
natural coenzyme
Mycobacterium tuberculosis
1.1.1.42
NADP+
natural coenzyme
Saccharomyces cerevisiae
1.1.1.42
NADP+
natural coenzyme
Sus scrofa
1.1.1.42
NADP+
natural coenzyme
Trypanosoma cruzi
1.1.1.42
NADPH
-
Citrobacter freundii
1.1.1.42
NADPH
-
Mycobacterium tuberculosis
1.1.1.42
NADPH
-
Trypanosoma cruzi
1.1.1.42
NADPH
-
Sus scrofa
1.1.1.42
NADPH
-
Saccharomyces cerevisiae
1.1.1.42
NADPH
-
Aspergillus niger
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.41
AMP
-
Saccharomyces cerevisiae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.41
NAD+
-
Aspergillus niger
1.1.1.41
NAD+
-
Cavia porcellus
1.1.1.41
NAD+
-
Columba livia
1.1.1.41
NAD+
-
Rattus norvegicus
1.1.1.41
NAD+
-
Bos taurus
1.1.1.41
NAD+
-
Pisum sativum
1.1.1.41
NAD+
-
Saccharomyces cerevisiae
1.1.1.41
NAD+
-
Homo sapiens
1.1.1.42
NADP+
natural coenzyme
Aspergillus niger
1.1.1.42
NADP+
natural coenzyme
Citrobacter freundii
1.1.1.42
NADP+
natural coenzyme
Mycobacterium tuberculosis
1.1.1.42
NADP+
natural coenzyme
Saccharomyces cerevisiae
1.1.1.42
NADP+
natural coenzyme
Sus scrofa
1.1.1.42
NADP+
natural coenzyme
Trypanosoma cruzi
1.1.1.42
NADPH
-
Citrobacter freundii
1.1.1.42
NADPH
-
Mycobacterium tuberculosis
1.1.1.42
NADPH
-
Trypanosoma cruzi
1.1.1.42
NADPH
-
Sus scrofa
1.1.1.42
NADPH
-
Saccharomyces cerevisiae
1.1.1.42
NADPH
-
Aspergillus niger
General Stability (protein specific)
EC Number
General Stability
Organism
1.1.1.42
instable in solutions of low ionic strength, stabilization by ammonium sulfate
Sus scrofa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.41
ATP
-
Bos taurus
1.1.1.41
CN-
-
Bos taurus
1.1.1.41
CN-
-
Pisum sativum
1.1.1.41
CN-
-
Saccharomyces cerevisiae
1.1.1.41
molybdate
-
Bos taurus
1.1.1.41
molybdate
-
Saccharomyces cerevisiae
1.1.1.41
N3-
-
Bos taurus
1.1.1.41
N3-
-
Saccharomyces cerevisiae
1.1.1.41
p-hydroxymercuribenzoate
-
Bos taurus
1.1.1.41
thiocyanate
-
Bos taurus
1.1.1.42
Ag+
decarboxylation of isocitrate and oxalosuccinate
Sus scrofa
1.1.1.42
Cu2+
-
Sus scrofa
1.1.1.42
Diphenylchloroarsine
-
Sus scrofa
1.1.1.42
isocitrate
competitive inhibition of decarboxylation of oxalosuccinate
Sus scrofa
1.1.1.42
p-chloromercuribenzoate
-
Sus scrofa
1.1.1.42
p-hydroxymercuribenzoate
decarboxylation of isocitrate and oxalosuccinate
Sus scrofa
1.1.1.42
Phenarsazines
-
Sus scrofa
1.1.1.42
Phenylmercuric nitrate
-
Sus scrofa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.41
0.11
-
D,L-isocitrate
-
Saccharomyces cerevisiae
1.1.1.41
0.45
-
D,L-isocitrate
-
Bos taurus
1.1.1.42
0.0026
-
D,L-isocitrate
-
Sus scrofa
1.1.1.42
0.0092
-
NADPH
-
Sus scrofa
1.1.1.42
0.13
-
2-oxoglutarate
-
Sus scrofa
1.1.1.42
0.56
-
oxalosuccinate
reductase
Sus scrofa
1.1.1.42
1.2
-
oxalosuccinate
decarboxylase
Sus scrofa
1.1.1.42
25
26
oxalosuccinate
decarboxylase
Sus scrofa
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.41
mitochondrion
-
Aspergillus niger
5739
-
1.1.1.41
mitochondrion
-
Cavia porcellus
5739
-
1.1.1.41
mitochondrion
-
Columba livia
5739
-
1.1.1.41
mitochondrion
-
Bos taurus
5739
-
1.1.1.41
mitochondrion
-
Rattus norvegicus
5739
-
1.1.1.41
mitochondrion
-
Pisum sativum
5739
-
1.1.1.41
mitochondrion
-
Saccharomyces cerevisiae
5739
-
1.1.1.41
mitochondrion
-
Homo sapiens
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.41
Mg2+
-
Aspergillus niger
1.1.1.41
Mg2+
-
Bos taurus
1.1.1.41
Mg2+
-
Pisum sativum
1.1.1.41
Mg2+
-
Saccharomyces cerevisiae
1.1.1.41
Mn2+
requires divalent cations
Bos taurus
1.1.1.41
Mn2+
requires divalent cations
Saccharomyces cerevisiae
1.1.1.41
Mn2+
requires divalent cations
Pisum sativum
1.1.1.42
Mg2+
absolute requirement for divalent cations
Aspergillus niger
1.1.1.42
Mg2+
absolute requirement for divalent cations
Sus scrofa
1.1.1.42
Mn2+
absolute requirement for divalent cations
Aspergillus niger
1.1.1.42
Mn2+
absolute requirement for divalent cations
Sus scrofa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
61000
64000
ultracentrifugation
Sus scrofa
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.41
isocitrate + NAD+
Cavia porcellus
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Homo sapiens
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Rattus norvegicus
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Saccharomyces cerevisiae
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Bos taurus
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Aspergillus niger
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Pisum sativum
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Columba livia
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.42
isocitrate + NADP+
Sus scrofa
-
2-oxoglutarate + NADPH + CO2
-
-
r
1.1.1.42
isocitrate + NADP+
Saccharomyces cerevisiae
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Aspergillus niger
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Mycobacterium tuberculosis
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Trypanosoma cruzi
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Citrobacter freundii
-
2-oxoglutarate + NADPH + CO2
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.41
heart
-
Cavia porcellus
-
1.1.1.41
heart
-
Bos taurus
-
1.1.1.41
heart
-
Rattus norvegicus
-
1.1.1.41
heart
-
Homo sapiens
-
1.1.1.41
muscle
breast muscle
Columba livia
-
1.1.1.41
muscle
-
Homo sapiens
-
1.1.1.41
placenta
-
Homo sapiens
-
1.1.1.42
heart
-
Sus scrofa
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
-
286749
Cavia porcellus
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
286749
Homo sapiens
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
286749
Rattus norvegicus
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
286749
Saccharomyces cerevisiae
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
286749
Bos taurus
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
286749
Aspergillus niger
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
286749
Pisum sativum
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
286749
Columba livia
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286749
Sus scrofa
2-oxoglutarate + NADPH + CO2
-
-
-
r
1.1.1.42
isocitrate + NADP+
-
286749
Saccharomyces cerevisiae
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286749
Aspergillus niger
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286749
Mycobacterium tuberculosis
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286749
Trypanosoma cruzi
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286749
Citrobacter freundii
2-oxoglutarate + NADPH + CO2
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.42
58.3
-
isocitrate
oxidative decarboxylation
Sus scrofa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.41
6.5
-
-
Bos taurus
1.1.1.41
7.5
-
-
Pisum sativum
1.1.1.41
7.5
-
-
Saccharomyces cerevisiae
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.41
6.5
8.5
-
Pisum sativum
1.1.1.41
6.5
8.5
-
Saccharomyces cerevisiae
1.1.1.42
5.6
8.5
-
Sus scrofa