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Literature summary extracted from

  • Bartholome, K.; Brdiczka, D.G.; Pette, D.
    Purification and properties of extra- and intramitochondrial malate dehydrogenase (NADP; decarboxylating) from pig heart (1972), Hoppe-Seyler's Z. Physiol. Chem., 353, 1487-1495.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.40 acetyl-CoA inhibition is much more pronounced in the mitochondrial enzyme than in the cytosolic enzyme and occurs at physiological acetyl-CoA concentrations Sus scrofa
1.1.1.40 malonyl-CoA inhibition of mitochondrial enzyme and cytosolic enzyme to a much lower extent than with acetyl-CoA Sus scrofa
1.1.1.40 Mg2+ mitochondrial enzyme, decarboxylation reaction, above 6 mM Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.40 0.00118
-
 NADP+ cytosolic enzyme Sus scrofa
1.1.1.40 0.00188
-
 NADP+ mitochondrial enzyme Sus scrofa
1.1.1.40 0.14
-
 L-malate cytosolic enzyme Sus scrofa
1.1.1.40 1.25
-
 L-malate mitochondrial enzyme Sus scrofa
1.1.1.40 10
-
 pyruvate cytosolic enzyme Sus scrofa
1.1.1.40 25
-
 pyruvate mitochondrial enzyme Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.40 cytosol 2 distinct enzyme forms are present in cytosol and in mitochondria Sus scrofa 5829
-
1.1.1.40 mitochondrion 2 distinct enzyme forms are present in cytosol and in mitochondria Sus scrofa 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.40 Mg2+ activity of cytosolic enzyme and mitochondrial enzyme is strictly dependent on Sus scrofa
1.1.1.40 Mg2+ optimal concentration: 6 mM for the decarboxylation of the mitochondrial enzyme, 20 mM for the cytosolic enzyme Sus scrofa
1.1.1.40 Mn2+ activity of cytosolic enzyme and mitochondrial enzyme is strictly dependent on Sus scrofa
1.1.1.40 Mn2+ optimal concentration is 15 mM for the cytosolic enzyme and 10 mM for the mitochondrial enzyme Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.40 216000
-
 cytosolic enzyme, density gradient centrifugation Sus scrofa
1.1.1.40 227000
-
 mitochondrial enzyme, density gradient centrifugation Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.40 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.40 2 distinct enzyme forms Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.40 cardiac muscle
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.40 additional information
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.40 (S)-malate + NADP+
-
 Sus scrofa pyruvate + CO2 + NADPH
-
 r
1.1.1.40 pyruvate + CO2 + NADPH
-
 Sus scrofa L-malate + NADP+
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.40 6.6
-
 mitochondrial enzyme, carboxylation Sus scrofa
1.1.1.40 7
-
 carboxylation and decarboxylation, cytosolic enzyme Sus scrofa
1.1.1.40 7.6
-
 cytosolic enzyme, decarboxylation Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.40 NADP+ cofactor Sus scrofa
1.1.1.40 NADPH cofactor Sus scrofa