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Literature summary extracted from

  • Rutter, W.J.; Lardy, H.A.
    Purification and properties of pigeon liver malic enzyme (1958), J. Biol. Chem., 233, 374-382.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.40 malic acid decreases heat stability at pH values above 5.0 Pigeon
1.1.1.40 Mg2+ or Mn2+ increase heat stability Pigeon

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.40 adenosine 2'-phosphate
-
 Pigeon
1.1.1.40 ADP
-
 Pigeon
1.1.1.40 arsenite weak Pigeon
1.1.1.40 Cu2+ 3 mM, weak Pigeon
1.1.1.40 diphenyliodonium chloride weak Pigeon
1.1.1.40 Hg2+ 0.0005 mM, almost complete inhibition Pigeon
1.1.1.40 iodoacetate weak Pigeon
1.1.1.40 NAD+ weak Pigeon
1.1.1.40 o-Iodosobenzoate strong Pigeon
1.1.1.40 p-mercuribenzoate strong Pigeon

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.40 0.016
-
 NADP+ pH 7.5 Pigeon
1.1.1.40 0.041
-
 L-malate pH 6.5 Pigeon
1.1.1.40 0.39
-
 L-malate pH 7.5 Pigeon
1.1.1.40 3.3
-
 L-malate pH 8.5 Pigeon

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.40 Co2+ less effective activation than Mn2+ Pigeon
1.1.1.40 Cu2+ weak activation Pigeon
1.1.1.40 Mg2+ promotes activity Pigeon
1.1.1.40 Mn2+ 0.004 mM, 50% of the maximal activity of the malic activity. 0.2 mM, 50% of the maximal activity of oxaloacetic decarboxylase Pigeon
1.1.1.40 Ni2+ weak activation Pigeon
1.1.1.40 Zn2+ weak activation Pigeon

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.40 Pigeon
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.40
-
 Pigeon

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.40 liver
-
 Pigeon
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.40 additional information
-
-
 Pigeon

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.40 (S)-malate + NADP+ the singly-ionized species is the substrate, doubly-ionized malate is unreactive Pigeon pyruvate + CO2 + NADPH
-
 r
1.1.1.40 Oxaloacetate
-
 Pigeon Pyruvate + CO2
-
 ?
1.1.1.40 pyruvate + CO2 + NADPH
-
 Pigeon L-malate + NADP+
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.40 additional information
-
 Mg2+ or Mn2+ increase heat stability Pigeon
1.1.1.40 additional information
-
 malic acid decreases heat stability at pH values above 5.0 Pigeon

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.40 7.2
-
 in presence of 0.1 mM malate Pigeon
1.1.1.40 8.5 9 in presence of 0.1 M malate Pigeon

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.40 NADP+ cofactor Pigeon
1.1.1.40 NADP+ stimulation of decarboxylation of oxaloacetate Pigeon
1.1.1.40 NADPH cofactor Pigeon