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Literature summary extracted from

  • Chen, F.; Okabe, Y.; Osano, K.; Tajima, S.
    Purification and characterization of an NAD-malic enzyme from Bradyrhizobium japonicum A1017 (1998), Appl. Environ. Microbiol., 64, 4073-4075.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.39 adenosine 5'-diphosphoglucose activates Bradyrhizobium japonicum
1.1.1.39 alpha-D-glucose 1-phosphate activation Bradyrhizobium japonicum
1.1.1.39 D-fructose 6-phosphate activation Bradyrhizobium japonicum
1.1.1.39 D-glucose 6-phosphate activation Bradyrhizobium japonicum
1.1.1.39 fructose 1,6-bisphosphate activates Bradyrhizobium japonicum
1.1.1.39 gamma-amino-n-butyrate slight activation Bradyrhizobium japonicum
1.1.1.39 hydroxy-n-butyrate slight activation Bradyrhizobium japonicum
1.1.1.39 Hydroxypyruvate slight activation Bradyrhizobium japonicum
1.1.1.39 succinate stimulates Bradyrhizobium japonicum
1.1.1.39 uridine 5'-diphosphoglucose activates Bradyrhizobium japonicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.39 2-Ketoglutarate
-
 Bradyrhizobium japonicum
1.1.1.39 AMP
-
 Bradyrhizobium japonicum
1.1.1.39 ATP
-
 Bradyrhizobium japonicum
1.1.1.39 CoA
-
 Bradyrhizobium japonicum
1.1.1.39 DL-isocitrate
-
 Bradyrhizobium japonicum
1.1.1.39 fumarate
-
 Bradyrhizobium japonicum
1.1.1.39 L-asparatate
-
 Bradyrhizobium japonicum
1.1.1.39 malonate
-
 Bradyrhizobium japonicum
1.1.1.39 phosphoenolpyruvate
-
 Bradyrhizobium japonicum
1.1.1.39 pyruvate
-
 Bradyrhizobium japonicum
1.1.1.39 Tartrate
-
 Bradyrhizobium japonicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.39 Mg2+ strict requirement for a divalent cation, maximal activation at 2 mM Bradyrhizobium japonicum
1.1.1.39 Mn2+ strict requirement for a divalent cation, maximal activation at 5 mM Bradyrhizobium japonicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.39 85000
-
 4 * 85000, SDS-PAGE Bradyrhizobium japonicum
1.1.1.39 388000
-
 gel filtration Bradyrhizobium japonicum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.39 Bradyrhizobium japonicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.39
-
 Bradyrhizobium japonicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.39 additional information
-
-
 Bradyrhizobium japonicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.39 malate + NAD+
-
 Bradyrhizobium japonicum pyruvate + CO2 + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.39 tetramer 4 * 85000, SDS-PAGE Bradyrhizobium japonicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.39 NAD+ cofactor Bradyrhizobium japonicum