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Literature summary extracted from

  • Eyzaguirre, J.; Cornwell, E.; Borie, G.; Ramirez, B.
    Two malic enzymes in Pseudomonas aeruginosa (1973), J. Bacteriol., 116, 215-221.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.40 NH4+ activates Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.39 0.52
-
 (S)-malate
-
 Pseudomonas aeruginosa
1.1.1.40 0.07
-
 NADP+
-
 Pseudomonas aeruginosa
1.1.1.40 2.7
-
 (S)-malate
-
 Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.39 Mg2+ bivalent metal ion required, Mn2+ is more effective than Mg2+ Pseudomonas aeruginosa
1.1.1.39 Mn2+ bivalent metal ion required, Mn2+ is more effective than Mg2+ Pseudomonas aeruginosa
1.1.1.40 K+ activates Pseudomonas aeruginosa
1.1.1.40 K+ Km: 0.7 mM Pseudomonas aeruginosa
1.1.1.40 Mg2+ divalent metal ion required, Mn2+ is more effective than Mg2+, Km: 0.34 mM Pseudomonas aeruginosa
1.1.1.40 Mn2+ Km: 0.004 mM Pseudomonas aeruginosa
1.1.1.40 Mn2+ divalent metal ion required, Mn2+ is more effective than Mg2+ Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.39 200000
-
 sucrose density gradient centrifugation Pseudomonas aeruginosa
1.1.1.39 270000
-
 gel filtration Pseudomonas aeruginosa
1.1.1.40 68000
-
 gel filtration Pseudomonas aeruginosa
1.1.1.40 90000
-
 sucrose density gradient centrifugation Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.39 Pseudomonas aeruginosa
-
-
-
1.1.1.40 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.40
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.39 (S)-malate + NADP+
-
 Pseudomonas aeruginosa pyruvate + CO2 + NADPH
-
 r
1.1.1.39 malate + NAD+
-
 Pseudomonas aeruginosa pyruvate + CO2 + NADH + H+
-
 r
1.1.1.40 (S)-malate + NADP+
-
 Pseudomonas aeruginosa pyruvate + CO2 + NADPH
-
 r
1.1.1.40 pyruvate + CO2 + NADPH
-
 Pseudomonas aeruginosa L-malate + NADP+
-
 r

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.39 NAD+ cofactor Pseudomonas aeruginosa
1.1.1.40 NADP+
-
 Pseudomonas aeruginosa