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Literature summary extracted from

  • Hatch, M.D.; Mau, S.L.; Kagawa, T.
    Properties of leaf NAD malic enzyme from plants with C4 pathway photosynthesis (1974), Arch. Biochem. Biophys., 165, 188-200.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.39 coenzyme A increases activity Panicum miliaceum
1.1.1.39 coenzyme A increases activity Atriplex spongiosa
1.1.1.39 coenzyme A increases activity Amaranthus edulis
1.1.1.39 SO42- activates Atriplex spongiosa

General Stability

EC Number General Stability Organism
1.1.1.39 freezing of crude enzyme extract treated on Sephadex G-25 destroys activity Atriplex spongiosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.39 (S)-malate above 10 mM Atriplex spongiosa
1.1.1.39 bicarbonate
-
 Amaranthus edulis
1.1.1.39 bicarbonate
-
 Atriplex spongiosa
1.1.1.39 HCO3-
-
 Atriplex spongiosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.39 0.3
-
 NAD+
-
 Atriplex spongiosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.39 Mg2+ no activation by Mg2+ Panicum miliaceum
1.1.1.39 Mg2+ no activation by Mg2+ Atriplex spongiosa
1.1.1.39 Mg2+ no activation by Mg2+ Amaranthus edulis
1.1.1.39 Mn2+ absolute requirement for Mn2+, no activation by Mg2+ Panicum miliaceum
1.1.1.39 Mn2+ absolute requirement for Mn2+, no activation by Mg2+ Atriplex spongiosa
1.1.1.39 Mn2+ absolute requirement for Mn2+, no activation by Mg2+ Amaranthus edulis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.39 300000
-
 gel filtration Atriplex spongiosa

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.39 Amaranthus edulis
-
-
-
1.1.1.39 Atriplex spongiosa
-
-
-
1.1.1.39 Panicum miliaceum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.39
-
 Atriplex spongiosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.39 leaf bundle sheath Panicum miliaceum
-
1.1.1.39 leaf bundle sheath Atriplex spongiosa
-
1.1.1.39 leaf bundle sheath Amaranthus edulis
-

Storage Stability

EC Number Storage Stability Organism
1.1.1.39 crude and partially purified enzyme retains activity for several months when stored as a protein suspension in 75% saturated (NH4)2SO4 solution Atriplex spongiosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.39 (S)-malate + NADP+ 15% of the activity with NAD+ Atriplex spongiosa pyruvate + CO2 + NADPH
-
 ?
1.1.1.39 malate + NAD+
-
 Panicum miliaceum pyruvate + CO2 + NADH + H+
-
 ?
1.1.1.39 malate + NAD+
-
 Atriplex spongiosa pyruvate + CO2 + NADH + H+
-
 ?
1.1.1.39 malate + NAD+
-
 Amaranthus edulis pyruvate + CO2 + NADH + H+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.39 6.9
-
 with NAD+, without activator Atriplex spongiosa
1.1.1.39 6.9
-
 activity with NADP+ Atriplex spongiosa
1.1.1.39 7.2
-
 with NAD+ plus CoA Atriplex spongiosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.39 NAD+ cofactor Panicum miliaceum
1.1.1.39 NAD+ cofactor Atriplex spongiosa
1.1.1.39 NAD+ cofactor Amaranthus edulis
1.1.1.39 NADP+ 15% of the activity with NAD+ Atriplex spongiosa