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Literature summary extracted from

  • Grover, S.D.; Canellas, P.F.; Wedding, R.T.
    Purification of NAD malic enzyme from potato and investigation of some physical and kinetic properties (1981), Arch. Biochem. Biophys., 209, 396-407.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.39 0.3
-
 NADP+ activation by Mn2+ Solanum tuberosum
1.1.1.39 0.5
-
 NAD+ activation by Mn2+ Solanum tuberosum
1.1.1.39 0.76
-
 (S)-malate with NAD+ and Mn2+ Solanum tuberosum
1.1.1.39 0.9
-
 NAD+ activation by Mg2+ Solanum tuberosum
1.1.1.39 1.7
-
 NADP+ with NADP+ as coenzyme Solanum tuberosum
1.1.1.39 4.2
-
 (S)-malate with NADP+ and Mn2+ Solanum tuberosum
1.1.1.39 12.3
-
 (S)-malate with NADP+ and Mg2+ Solanum tuberosum
1.1.1.39 14
-
 (S)-malate with NAD+ and Mg2+ Solanum tuberosum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.39 Mg2+ activation by Mg2+ or Mn2+ Solanum tuberosum
1.1.1.39 Mn2+ activation by Mn2+ or Mg2+ Solanum tuberosum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.39 58000
-
 alpha4beta4, 4 * 61000 + 4 * 58000, SDS-PAGE Solanum tuberosum
1.1.1.39 61000
-
 alpha4beta4, 4 * 61000 + 4 * 58000, SDS-PAGE Solanum tuberosum
1.1.1.39 490000
-
 gel filtration in presence of 5 mM dithiothreitol and 2 mM MgCl2 Solanum tuberosum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.39 Solanum tuberosum
-
 var. Chieftan
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.39
-
 Solanum tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.39 tuber
-
 Solanum tuberosum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.39 72.5
-
-
 Solanum tuberosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.39 (S)-malate + NADP+
-
 Solanum tuberosum pyruvate + CO2 + NADPH
-
 ?
1.1.1.39 malate + NAD+
-
 Solanum tuberosum pyruvate + CO2 + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.39 octamer alpha4beta4, 4 * 61000 + 4 * 58000, SDS-PAGE Solanum tuberosum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.39 617
-
 (S)-malate
-
 Solanum tuberosum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.39 6.4
-
 activity with NADP+ and Mg2+ Solanum tuberosum
1.1.1.39 6.5
-
 activity with NADP+ and Mn2+ Solanum tuberosum
1.1.1.39 6.8
-
 activity with NAD+ Solanum tuberosum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.39 NAD+ cofactor Solanum tuberosum
1.1.1.39 NADP+
-
 Solanum tuberosum