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Literature summary extracted from

  • Wedding, R.T.; Black, M.K.
    Physical and kinetic properties and regulation of the NAD malic enzyme purified from leaves of Crassula argentea (1983), Plant Physiol., 72, 1021-1028.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.39 1-N6-etheno-CoA activates Crassula argentea
1.1.1.39 AMP competitive activation Crassula argentea
1.1.1.39 coenzyme A activates Crassula argentea
1.1.1.39 fructose 1,6-bisphosphate activates Crassula argentea
1.1.1.39 fumarate fumarate2- is a strong activator Crassula argentea
1.1.1.39 phosphoenolpyruvate activates at low concentrations, decativation at high concentrations Crassula argentea

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.39 citrate competitive Crassula argentea
1.1.1.39 Cl-
-
 Crassula argentea
1.1.1.39 NADH product inhibition Crassula argentea
1.1.1.39 phosphoenolpyruvate activates at low concentrations, deactivation at high concentrations Crassula argentea
1.1.1.39 pyruvate product inhibition Crassula argentea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.39 0.12
-
 NADH activation by Mn2+ Crassula argentea
1.1.1.39 0.59
-
 (S)-malate activation by Mn2+ Crassula argentea
1.1.1.39 0.77
-
 NAD+ activation by Mg2+ Crassula argentea
1.1.1.39 0.8
-
 (S)-malate activation by Mn2+ Crassula argentea
1.1.1.39 6.03
-
 (S)-malate activation by Mg2+ Crassula argentea
1.1.1.39 13.48
-
 CO2 activation by Mg2+ Crassula argentea
1.1.1.39 15.03
-
 pyruvate activation by Mn2+ Crassula argentea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.39 Mg2+ uses Mg2+ or Mn2+ as the required divalent cation Crassula argentea
1.1.1.39 Mn2+ divalent cation required, Mg2+ or Mn2+ Crassula argentea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.39 119000
-
 dimer, disc gel electrophoresis Crassula argentea
1.1.1.39 231000
-
 tetramer, disc gel electrophoresis Crassula argentea
1.1.1.39 350000
-
 non-denaturing PAGE Crassula argentea
1.1.1.39 491000
-
 tetramer, disc gel electrophoresis Crassula argentea

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.39 Crassula argentea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.39
-
 Crassula argentea

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.39 (S)-malate + NAD+ = pyruvate + CO2 + NADH + H+ sequential mechanism Crassula argentea
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.39 leaf
-
 Crassula argentea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.39 35.4
-
-
 Crassula argentea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.39 (S)-malate + NADP+ 14% of the activity with NAD+ Crassula argentea pyruvate + CO2 + NADPH
-
 ?
1.1.1.39 malate + NAD+ activity of the reverse reaction is 1.5% of that of the forward reaction Crassula argentea pyruvate + CO2 + NADH + H+
-
 r
1.1.1.39 pyruvate + CO2 + NADH activity is 1.5% of the decarboxylation of (S)-malate Crassula argentea (S)-malate + NAD+
-
 r

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.39 NAD+ cofactor Crassula argentea
1.1.1.39 NADP+ cofactor Crassula argentea
1.1.1.39 NADP+ 14% of the activity with NAD+ Crassula argentea