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Literature summary extracted from

  • Canellas, P.F.; Wedding, R.T.
    Kinetic properties of NAD malic enzyme from cauliflower (1984), Arch. Biochem. Biophys., 229, 414-425.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.39 KCNO
-
 Brassica oleracea
1.1.1.39 NEM
-
 Brassica oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.39 0.47
-
 NAD+ with 30 mM Mg2+ Brassica oleracea
1.1.1.39 0.48
-
 NAD+ with 8 mM Mg2+ Brassica oleracea
1.1.1.39 0.5
-
 NAD+ with 80 mM Mg2+ Brassica oleracea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.39 Co2+ can replace Mg2+ in activation. Km: 0.018 mM Brassica oleracea
1.1.1.39 Mg2+ no decarboxylation of malate in absence of either Mg2+ or NAD+. Km: 0.04 mM Brassica oleracea
1.1.1.39 Mn2+ can replace Mg2+ in activation Brassica oleracea

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.39 Brassica oleracea
-
 var. botrytis
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.39
-
 Brassica oleracea

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.39 (S)-malate + NAD+ = pyruvate + CO2 + NADH + H+ sequential mechanism, each of the substrate pairs binds randomly to the enzyme Brassica oleracea
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.39 floret
-
 Brassica oleracea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.39 malate + NAD+ no decarboxylation of malate in absence of either Mg2+ or NAD+ Brassica oleracea pyruvate + CO2 + NADH + H+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.39 NAD+ cofactor Brassica oleracea