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Literature summary extracted from

  • Wise, D.J.; Anderson, C.D.; Anderson, B.M.
    Purification and kinetic characterization of Haemophilus parasuis malate dehydrogenase (1997), Arch. Biochem. Biophys., 344, 176-183.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.37 agriculture swine respiratory pathogen is the etiological agent of Glaesser's disease, swine industry economic losses worldwide Glaesserella parasuis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.37 3-Aminopyridine adenine dinucleotide
-
 Glaesserella parasuis
1.1.1.37 3-methylpyridine adenine dinucleotide
-
 Glaesserella parasuis
1.1.1.37 3-pyridylacetonitrile adenine dinucleotide
-
 Glaesserella parasuis
1.1.1.37 3-pyridylcarbinol adenine dinucleotide
-
 Glaesserella parasuis
1.1.1.37 adenosine
-
 Glaesserella parasuis
1.1.1.37 adenosine 5'-diphosphoribose
-
 Glaesserella parasuis
1.1.1.37 ADP
-
 Glaesserella parasuis
1.1.1.37 AMP
-
 Glaesserella parasuis
1.1.1.37 ATP
-
 Glaesserella parasuis
1.1.1.37 Hydroxymalonate
-
 Glaesserella parasuis
1.1.1.37 NAD+
-
 Glaesserella parasuis
1.1.1.37 NADH product inhibition Glaesserella parasuis
1.1.1.37 pyridine adenine dinucleotide
-
 Glaesserella parasuis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.37 0.0165
-
 NADH
-
 Glaesserella parasuis
1.1.1.37 0.0667
-
 3-acetylpyridine adenine dinucleotide
-
 Glaesserella parasuis
1.1.1.37 0.072
-
 oxaloacetate
-
 Glaesserella parasuis
1.1.1.37 0.117
-
 NAD+
-
 Glaesserella parasuis
1.1.1.37 0.132
-
 Thionicotinamide adenine dinucleotide
-
 Glaesserella parasuis
1.1.1.37 0.19
-
 pyridine 3-aldehyde adenine dinucleotide
-
 Glaesserella parasuis
1.1.1.37 0.25
-
 nicotinamide hypoxanthine dinucleotide
-
 Glaesserella parasuis
1.1.1.37 0.55
-
 malate
-
 Glaesserella parasuis
1.1.1.37 1.464
-
 nicotinamide 1-N6-ethenoadenine dinucleotide
-
 Glaesserella parasuis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.37 cytoplasm
-
 Glaesserella parasuis 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.37 34600
-
 2 * 34600, SDS-PAGE Glaesserella parasuis
1.1.1.37 68400
-
 gel filtration Glaesserella parasuis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.37 malate + NAD+ Glaesserella parasuis
-
 oxaloacetate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.37 Glaesserella parasuis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.37
-
 Glaesserella parasuis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.37 222
-
-
 Glaesserella parasuis

Storage Stability

EC Number Storage Stability Organism
1.1.1.37 -15°C, in 10 mM potassium phosphate buffer, pH 7.5, containing 0.1 M KCl, stable for at least 3 months with no apparent loss of activity Glaesserella parasuis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.37 (S)-malate + NAD+
-
 Glaesserella parasuis oxaloacetate + NADH + H+
-
 r
1.1.1.37 3-acetylpyridine adenine dinucleotide + L-malate
-
 Glaesserella parasuis ?
-
 ?
1.1.1.37 malate + NAD+
-
 Glaesserella parasuis oxaloacetate + NADH + H+
-
 r
1.1.1.37 nicotinamide 1-N6-ethenoadenine dinucleotide + L-malate
-
 Glaesserella parasuis ?
-
 ?
1.1.1.37 nicotinamide hypoxanthine dinucleotide + L-malate
-
 Glaesserella parasuis ?
-
 ?
1.1.1.37 oxaloacetate + NADH
-
 Glaesserella parasuis L-malate + NAD+
-
 r
1.1.1.37 pyridine 3-aldehyde adenine dinucleotide + L-malate
-
 Glaesserella parasuis ?
-
 ?
1.1.1.37 thionicotinamide adenine dinucleotide + L-malate
-
 Glaesserella parasuis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.37 dimer 2 * 34600, SDS-PAGE Glaesserella parasuis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.37 NAD+ NADP+ does not serve as a coenzyme Glaesserella parasuis