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Literature summary extracted from

  • Lindbladh, C.; Rault, M.; Hagglund, C.; Small, W.C.; Mosbach, K.; Buelow, L.; Evans, C.; Srere, P.A.
    Preparation and kinetic characterization of a fusion protein of yeast mitochondrial citrate synthase and malate dehydrogenase (1994), Biochemistry, 33, 11692-11698.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.37 0.0094
-
 oxaloacetate fusion protein of MDH with mitochondrial citrate synthase Saccharomyces cerevisiae
1.1.1.37 0.0136
-
 NADH fusion protein of MDH with mitochondrial citrate synthase Saccharomyces cerevisiae
1.1.1.37 0.014
-
 L-malate fusion protein of MDH with mitochondrial citrate synthase Saccharomyces cerevisiae
1.1.1.37 0.023
-
 oxaloacetate
-
 Saccharomyces cerevisiae
1.1.1.37 0.028
-
 L-malate
-
 Saccharomyces cerevisiae
1.1.1.37 0.03
-
 NADH
-
 Saccharomyces cerevisiae
1.1.1.37 0.11
-
 NAD+ fusion protein of MDH with mitochondrial citrate synthase Saccharomyces cerevisiae
1.1.1.37 0.14
-
 L-malate fusion protein of MDH with mitochondrial citrate synthase Saccharomyces cerevisiae
1.1.1.37 0.28
-
 L-malate
-
 Saccharomyces cerevisiae
1.1.1.37 0.3
-
 NAD+
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.37 170000
-
 fusion protein of MDH with mitochondrial citrate synthase, gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.37 malate + NAD+ Saccharomyces cerevisiae
-
 oxaloacetate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.37 Saccharomyces cerevisiae
-
 yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.37 fusion protein of MDH with mitochondrial citrate synthase Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.37 (S)-malate + NAD+
-
 Saccharomyces cerevisiae oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Saccharomyces cerevisiae oxaloacetate + NADH + H+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Saccharomyces cerevisiae L-malate + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.37 dimer 2 * 90000-95000, fusion protein of MDH with mitochondrial citrate synthase, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
1.1.1.37 MDH
-
 Saccharomyces cerevisiae