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Literature summary extracted from

  • Sprott, G.D.; McKellar, R.C.; Shaw, K.M.; Giroux, J.; Martin, W.G.
    Properties of malate dehydrogenase isolated from Methanospirillum hungatii (1978), Can. J. Microbiol., 25, 192-200.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.37 2',3'-Cyclic AMP
-
 Methanospirillum hungatei
1.1.1.37 acetyl-CoA
-
 Methanospirillum hungatei
1.1.1.37 ADP
-
 Methanospirillum hungatei
1.1.1.37 alpha-ketoglutarate end-product inhibition Methanospirillum hungatei
1.1.1.37 ATP
-
 Methanospirillum hungatei
1.1.1.37 L-malate
-
 Methanospirillum hungatei
1.1.1.37 L-malate
-
 Sus scrofa
1.1.1.37 NADH
-
 Methanospirillum hungatei
1.1.1.37 NADH
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.37 0.027
-
 oxaloacetate
-
 Methanospirillum hungatei
1.1.1.37 0.081
-
 NADH
-
 Methanospirillum hungatei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.37 61700
-
 gel filtration Methanospirillum hungatei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.37 malate + NAD+ Escherichia coli
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Sus scrofa
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Saccharomyces cerevisiae
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Methanothermobacter thermautotrophicus
-
 oxaloacetate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.37 Escherichia coli
-
-
-
1.1.1.37 Methanospirillum hungatei
-
 type strain JF1, strain GP1
-
1.1.1.37 Methanothermobacter thermautotrophicus
-
-
-
1.1.1.37 Saccharomyces cerevisiae
-
 yeast
-
1.1.1.37 Sus scrofa
-
 pig
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.37
-
 Methanospirillum hungatei

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.37 heart
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.37 1.24
-
-
 Methanospirillum hungatei

Storage Stability

EC Number Storage Stability Organism
1.1.1.37 4°C, storage in Tris-HCl, 0.01 M, pH 8, 0.2 M NaCl results in a 96% loss of activity within 48 h, loss of activity can be prevented completely for at least 4 weeks by storage in the same buffer, containing 1 M NaCl, under the latter conditions the enzyme is inactive, activity is completely restored upon dilution of the salt to 20 mM Methanospirillum hungatei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.37 (S)-malate + NAD+
-
 Escherichia coli oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Sus scrofa oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Saccharomyces cerevisiae oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Methanothermobacter thermautotrophicus oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Escherichia coli oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Sus scrofa oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Saccharomyces cerevisiae oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Methanothermobacter thermautotrophicus oxaloacetate + NADH + H+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Escherichia coli L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Sus scrofa L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Saccharomyces cerevisiae L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Methanothermobacter thermautotrophicus L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Methanospirillum hungatei L-malate + NAD+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.37 8
-
-
 Methanospirillum hungatei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.37 6.5 9 no activity remains below pH 5.5 and above pH 11 Methanospirillum hungatei