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Literature summary extracted from

  • Teague, W.M.; Henney, H.R.
    Purification and properties of cytoplasmic and mitochondria malate dehydrogenase of Physarum polycephalum (1973), J. Bacteriol., 116, 673-684.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.37 L-malate
-
 Gallus sp.
1.1.1.37 L-malate
-
 Physarum polycephalum
1.1.1.37 oxaloacetate
-
 Drosophila virilis
1.1.1.37 oxaloacetate
-
 Phycomyces blakesleeanus
1.1.1.37 oxaloacetate
-
 Physarum polycephalum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.37 0.048
-
 NADH s-MDH Physarum polycephalum
1.1.1.37 0.06
-
 oxaloacetate s-MDH Physarum polycephalum
1.1.1.37 0.074
-
 oxaloacetate m-MDH Physarum polycephalum
1.1.1.37 0.19
-
 NAD+ m-MDH Physarum polycephalum
1.1.1.37 0.2
-
 NADH m-MDH Physarum polycephalum
1.1.1.37 0.75
-
 NAD+ s-MDH Physarum polycephalum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.37 cytoplasm
-
 Saccharomyces cerevisiae 5737
-
1.1.1.37 cytoplasm
-
 Bos taurus 5737
-
1.1.1.37 cytoplasm
-
 Neurospora crassa 5737
-
1.1.1.37 cytoplasm
-
 Phycomyces blakesleeanus 5737
-
1.1.1.37 cytoplasm
-
 Spinacia oleracea 5737
-
1.1.1.37 cytoplasm
-
 Ascaris suum 5737
-
1.1.1.37 cytoplasm
-
 Physarum polycephalum 5737
-
1.1.1.37 cytoplasm
-
 Fundulus heteroclitus 5737
-
1.1.1.37 cytoplasm
-
 Gallus sp. 5737
-
1.1.1.37 cytoplasm
-
 Drosophila virilis 5737
-
1.1.1.37 cytoplasm
-
 Thunnus albacares 5737
-
1.1.1.37 cytosol
-
 Saccharomyces cerevisiae 5829
-
1.1.1.37 cytosol
-
 Bos taurus 5829
-
1.1.1.37 cytosol
-
 Physarum polycephalum 5829
-
1.1.1.37 cytosol
-
 Gallus sp. 5829
-
1.1.1.37 mitochondrion
-
 Saccharomyces cerevisiae 5739
-
1.1.1.37 mitochondrion
-
 Bos taurus 5739
-
1.1.1.37 mitochondrion
-
 Neurospora crassa 5739
-
1.1.1.37 mitochondrion
-
 Spinacia oleracea 5739
-
1.1.1.37 mitochondrion
-
 Ascaris suum 5739
-
1.1.1.37 mitochondrion
-
 Physarum polycephalum 5739
-
1.1.1.37 mitochondrion
-
 Fundulus heteroclitus 5739
-
1.1.1.37 mitochondrion
-
 Gallus sp. 5739
-
1.1.1.37 mitochondrion
-
 Drosophila virilis 5739
-
1.1.1.37 mitochondrion
-
 Thunnus albacares 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.37 malate + NAD+ Saccharomyces cerevisiae
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Bos taurus
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Neurospora crassa
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Phycomyces blakesleeanus
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Spinacia oleracea
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Zea mays
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Ascaris suum
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Auxenochlorella pyrenoidosa
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Physarum polycephalum
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Citrus sp.
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Strongylocentrotus purpuratus
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Fundulus heteroclitus
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Gallus sp.
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Drosophila virilis
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Thunnus albacares
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Opuntia ficus-indica
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Salmo sp.
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Tritia obsoleta
-
 oxaloacetate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.37 Ascaris suum
-
-
-
1.1.1.37 Auxenochlorella pyrenoidosa
-
-
-
1.1.1.37 Bos taurus
-
-
-
1.1.1.37 Citrus sp.
-
 lemon fruit
-
1.1.1.37 Drosophila virilis
-
-
-
1.1.1.37 Fundulus heteroclitus
-
 teleost
-
1.1.1.37 Gallus sp.
-
 chicken
-
1.1.1.37 Neurospora crassa
-
-
-
1.1.1.37 Opuntia ficus-indica
-
 cactus
-
1.1.1.37 Phycomyces blakesleeanus
-
-
-
1.1.1.37 Physarum polycephalum
-
-
-
1.1.1.37 Saccharomyces cerevisiae
-
 yeast
-
1.1.1.37 Salmo sp.
-
 salmonid fish
-
1.1.1.37 Spinacia oleracea
-
 spinach
-
1.1.1.37 Strongylocentrotus purpuratus
-
 sea urchin
-
1.1.1.37 Thunnus albacares
-
 tuna
-
1.1.1.37 Tritia obsoleta
-
 marine snail
-
1.1.1.37 Zea mays
-
 maize
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.37 2 isoenzymes, S-MDH and M-MDH Physarum polycephalum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.37 endosperm
-
 Zea mays
-
1.1.1.37 kidney
-
 Bos taurus
-
1.1.1.37 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.37 774
-
-
 Physarum polycephalum

Storage Stability

EC Number Storage Stability Organism
1.1.1.37 0°C, purified enzymes are stable for up to 2 months in 0.05 M potassium phosphate buffer, pH 7.5 containing 0.001 M 2-mercaptoethanol Physarum polycephalum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.37 (S)-malate + NAD+
-
 Saccharomyces cerevisiae oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Bos taurus oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Neurospora crassa oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Phycomyces blakesleeanus oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Spinacia oleracea oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Zea mays oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Ascaris suum oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Auxenochlorella pyrenoidosa oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Physarum polycephalum oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Citrus sp. oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Strongylocentrotus purpuratus oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Fundulus heteroclitus oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Gallus sp. oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Drosophila virilis oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Thunnus albacares oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Opuntia ficus-indica oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Salmo sp. oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Tritia obsoleta oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Saccharomyces cerevisiae oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Bos taurus oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Neurospora crassa oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Phycomyces blakesleeanus oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Spinacia oleracea oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Zea mays oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Ascaris suum oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Auxenochlorella pyrenoidosa oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Physarum polycephalum oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Citrus sp. oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Strongylocentrotus purpuratus oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Fundulus heteroclitus oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Gallus sp. oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Drosophila virilis oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Thunnus albacares oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Opuntia ficus-indica oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Salmo sp. oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Tritia obsoleta oxaloacetate + NADH + H+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Saccharomyces cerevisiae L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Bos taurus L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Neurospora crassa L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Phycomyces blakesleeanus L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Spinacia oleracea L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Zea mays L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Ascaris suum L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Auxenochlorella pyrenoidosa L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Physarum polycephalum L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Citrus sp. L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Strongylocentrotus purpuratus L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Fundulus heteroclitus L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Gallus sp. L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Drosophila virilis L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Thunnus albacares L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Opuntia ficus-indica L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Salmo sp. L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Tritia obsoleta L-malate + NAD+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.37 s-MDH
-
 Physarum polycephalum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.37 51
-
 m-MDH, activity reduced to 50% Physarum polycephalum
1.1.1.37 55
-
 s-MDH, activity reduced to 50% Physarum polycephalum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.37 7.6
-
 oxaloacetate reduction, s-MDH Bos taurus
1.1.1.37 7.6
-
 oxaloacetate reduction, s-MDH Gallus sp.
1.1.1.37 7.6
-
 oxaloacetate reduction, both isoenzymes Physarum polycephalum
1.1.1.37 7.8
-
 oxaloacetate reduction, m-MDH Bos taurus
1.1.1.37 7.8
-
 oxaloacetate reduction, m-MDH Gallus sp.
1.1.1.37 10
-
-
 Thunnus albacares
1.1.1.37 10
-
 malate oxidation Physarum polycephalum