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Literature summary extracted from

  • Amos, D.A.; McInerney, M.J.
    Formation of D-3-hydroxybutyryl-coenzyme A by an acetoacetyl A reductase in Syntrophomonas wolfei subsp. wolfei (1993), Arch. Microbiol., 159, 16-20.
No PubMed abstract available

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.36 soluble
-
 Zoogloea ramigera
-
-
1.1.1.36 soluble
-
 Syntrophomonas wolfei
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.36 acetoacetyl-CoA + NADH + H+ Syntrophomonas wolfei PHA-synthesis D-3-hydroxybutyryl-CoA + NAD+
-
 ir
1.1.1.36 acetoacetyl-CoA + NADPH + H+ Syntrophomonas wolfei
-
 D-3-hydroxybutyryl-CoA + NADP+
-
 ?
1.1.1.36 acetoacetyl-CoA + NADPH + H+ Zoogloea ramigera PHB synthesis D-3-hydroxybutyryl-CoA + NADP+
-
 ?
1.1.1.36 acetoacetyl-CoA + NADPH + H+ Cupriavidus necator PHB synthesis D-3-hydroxybutyryl-CoA + NADP+
-
 ?
1.1.1.36 acetoacetyl-CoA + NADPH + H+ Azotobacter beijerinckii PHB synthesis D-3-hydroxybutyryl-CoA + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.36 Azotobacter beijerinckii
-
-
-
1.1.1.36 Cupriavidus necator
-
-
-
1.1.1.36 Syntrophomonas wolfei
-
 ssp. Wolfei
-
1.1.1.36 Zoogloea ramigera
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.36 ammonium sulfate fractionation, ion exchange, hydroxyapatite, affinity chromatography Syntrophomonas wolfei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.36 additional information
-
 no activity at pH 8.0 Syntrophomonas wolfei
1.1.1.36 4.82
-
 NADPH Syntrophomonas wolfei
1.1.1.36 10.6
-
 NADH Syntrophomonas wolfei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.36 acetoacetyl-CoA + NADH + H+ PHA-synthesis Syntrophomonas wolfei D-3-hydroxybutyryl-CoA + NAD+
-
 ir
1.1.1.36 acetoacetyl-CoA + NADPH + H+
-
 Zoogloea ramigera (3R)-3-hydroxybutyryl-CoA + NADP+
-
 ?
1.1.1.36 acetoacetyl-CoA + NADPH + H+
-
 Azotobacter beijerinckii (3R)-3-hydroxybutyryl-CoA + NADP+
-
 ?
1.1.1.36 acetoacetyl-CoA + NADPH + H+
-
 Syntrophomonas wolfei D-3-hydroxybutyryl-CoA + NADP+
-
 ?
1.1.1.36 acetoacetyl-CoA + NADPH + H+ PHB synthesis Zoogloea ramigera D-3-hydroxybutyryl-CoA + NADP+
-
 ?
1.1.1.36 acetoacetyl-CoA + NADPH + H+ PHB synthesis Cupriavidus necator D-3-hydroxybutyryl-CoA + NADP+
-
 ?
1.1.1.36 acetoacetyl-CoA + NADPH + H+ PHB synthesis Azotobacter beijerinckii D-3-hydroxybutyryl-CoA + NADP+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.36 8.1
-
 reduction of acetoacetyl-CoA Zoogloea ramigera

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.36 8
-
 no activity Syntrophomonas wolfei

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.36 NADH
-
 Zoogloea ramigera
1.1.1.36 NADH
-
 Syntrophomonas wolfei
1.1.1.36 NADH appreciable activity Cupriavidus necator
1.1.1.36 NADH appreciable activity Azotobacter beijerinckii
1.1.1.36 NADP+
-
 Zoogloea ramigera
1.1.1.36 NADP+
-
 Azotobacter beijerinckii
1.1.1.36 NADP+
-
 Syntrophomonas wolfei
1.1.1.36 NADPH
-
 Syntrophomonas wolfei
1.1.1.36 NADPH specific for Zoogloea ramigera
1.1.1.36 NADPH preferentially Cupriavidus necator
1.1.1.36 NADPH preferentially Azotobacter beijerinckii