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Literature summary extracted from

  • Ploux, O.; Masamune, S.; Walsh, C.T.
    The NADPH-linked acetoacetyl-CoA reductase from Zoogloea ramigera. Characterization and mechanistic studies of the cloned enzyme over-produced in Escherichia coli (1988), Eur. J. Biochem., 174, 177-182.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.36 expressed in Escherichia coli JM155 Zoogloea ramigera

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.36 acetoacetyl-CoA above 0.01 mM Zoogloea ramigera
1.1.1.36 propionylacetyl-CoA
-
 Zoogloea ramigera

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.36 0.002
-
 acetoacetyl-CoA
-
 Zoogloea ramigera
1.1.1.36 0.002
-
 propionylacetyl-CoA
-
 Zoogloea ramigera
1.1.1.36 0.01
-
 butyrylacetyl-CoA
-
 Zoogloea ramigera
1.1.1.36 0.02 0.021 NADPH cosubstrate acetoacetyl-CoA Zoogloea ramigera
1.1.1.36 0.026
-
 D-3-hydroxybutyryl-CoA
-
 Zoogloea ramigera
1.1.1.36 0.74
-
 2-methylacetoacetyl-CoA
-
 Zoogloea ramigera
1.1.1.36 0.99
-
 Acetoacetyl-S-(D-pantetheine)11-pivalate
-
 Zoogloea ramigera

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.36 soluble
-
 Zoogloea ramigera
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.36 24000
-
 4 * 24000 SDS-PAGE Zoogloea ramigera
1.1.1.36 25200
-
 4 * 25200 gene sequence Zoogloea ramigera
1.1.1.36 100000
-
 gel filtration Zoogloea ramigera

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.36 acetoacetyl-CoA + NADPH Zoogloea ramigera biosynthesis of poly(D-3-hydroxybutyrate) ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.36 Zoogloea ramigera
-
 gene cloned in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.36 DEAE-Sepharose chromatography, Blue gel column Zoogloea ramigera

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.36 608
-
-
 Zoogloea ramigera

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.36 2-methylacetoacetyl-CoA + NADPH
-
 Zoogloea ramigera 2-methyl-3-hydroxybutyryl-CoA + NADP+
-
 ?
1.1.1.36 acetoacetyl-CoA + NADPH biosynthesis of poly(D-3-hydroxybutyrate) Zoogloea ramigera ?
-
 ?
1.1.1.36 acetoacetyl-CoA + NADPH + H+
-
 Zoogloea ramigera (3R)-3-hydroxybutyryl-CoA + NADP+
-
 r
1.1.1.36 acetoacetyl-S-(D-pantetheine)11-pivalate + NADPH
-
 Zoogloea ramigera 3-hydroxybutyryl-S-(D-pantetheine)11-pivalate + NADP+
-
 ?
1.1.1.36 butyrylacetyl-CoA + NADPH
-
 Zoogloea ramigera 3-hydroxyhexanoyl-CoA + NADP+
-
 ?
1.1.1.36 propionylacetyl-CoA + NADPH
-
 Zoogloea ramigera 3-hydroxypentanoyl-CoA + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.36 tetramer 4 * 25200 gene sequence Zoogloea ramigera
1.1.1.36 tetramer 4 * 24000 SDS-PAGE Zoogloea ramigera

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.36 9
-
 butyrylacetyl-CoA
-
 Zoogloea ramigera
1.1.1.36 27
-
 2-methylacetoacetyl-CoA
-
 Zoogloea ramigera
1.1.1.36 90
-
 D-3-hydroxybutyryl-CoA
-
 Zoogloea ramigera
1.1.1.36 124
-
 propionylacetyl-CoA
-
 Zoogloea ramigera
1.1.1.36 267
-
 Acetoacetyl-S-(D-pantetheine)11-pivalate
-
 Zoogloea ramigera
1.1.1.36 300
-
 acetoacetyl-CoA
-
 Zoogloea ramigera

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.36 NADPH specific for Zoogloea ramigera
1.1.1.36 NADPH B-specific, i.e. transfer of pro-S-hydrogen from NADPH to acetoacetyl-CoA Zoogloea ramigera
1.1.1.36 NADPH absolute specificity Zoogloea ramigera