EC Number | General Stability | Organism |
---|---|---|
1.1.1.36 | glycerol stabilizes during purification | Zoogloea ramigera |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.36 | acetoacetyl-CoA | markedly at concentrations above 0.005 mM and NADPH 0.01 mM, less inhibition when NADPH increases from 0.01 to 1 mM | Zoogloea ramigera |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.36 | 25500 | - |
4 * 25500, SDS-PAGE | Zoogloea ramigera |
1.1.1.36 | 92000 | - |
gel electrophoresis | Zoogloea ramigera |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
1.1.1.36 | Glycerol | during purification | Zoogloea ramigera |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.36 | Pigeon | - |
- |
- |
1.1.1.36 | Zoogloea ramigera | - |
I-16-M | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.36 | ammonium sulfate fractionation, DEAE-Sephasrose CL-6B, hydroxyapatite, Red-Sepharose CL-6B chromatography | Zoogloea ramigera |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.36 | liver | - |
Zoogloea ramigera | - |
1.1.1.36 | liver | - |
Pigeon | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.36 | 412 | - |
- |
Zoogloea ramigera |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.36 | -20°C, 10 mM Tris/HCl buffer, pH 7.5, 10 mM 2-mercaptoethanol, 50% glycerol, several months | Zoogloea ramigera |
1.1.1.36 | 0°C and -20°C, 10 mM Tris/HCl buffer, pH 7.5, 10 mM 2-mercaptoethanol, rather unstable | Zoogloea ramigera |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.36 | acetoacetyl-CoA + NADPH + H+ | - |
Zoogloea ramigera | (3R)-3-hydroxybutyryl-CoA + NADP+ | - |
r | |
1.1.1.36 | acetoacetyl-CoA + NADPH + H+ | - |
Pigeon | (3R)-3-hydroxybutyryl-CoA + NADP+ | - |
r | |
1.1.1.36 | D-3-hydroxybutyryl-CoA + NADP+ | - |
Zoogloea ramigera | acetoacetyl-CoA + NADPH | - |
r | |
1.1.1.36 | additional information | - |
Pigeon | ? | - |
? | |
1.1.1.36 | additional information | poor substrate: D-3-hydroxyvaleryl-CoA | Zoogloea ramigera | ? | - |
? | |
1.1.1.36 | additional information | not: L-3-hydroxyacyl-CoA | Zoogloea ramigera | ? | - |
? | |
1.1.1.36 | additional information | not: D-3-hydroxyacyl-CoA's with chain length exceeding 6 | Zoogloea ramigera | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.36 | tetramer | 4 * 25500, SDS-PAGE | Zoogloea ramigera |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.36 | 30 | - |
- |
Zoogloea ramigera |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.36 | NADP+ | - |
Zoogloea ramigera | |
1.1.1.36 | NADP+ | - |
Pigeon | |
1.1.1.36 | NADPH | specific for | Zoogloea ramigera | |
1.1.1.36 | NADPH | specific for | Pigeon |