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Literature summary extracted from

  • Osumi, T.; Hashimoto, T.
    Purification and properties of mitochondrial and peroxisomal 3-hydroxyacyl-CoA dehydrogenase from rat liver (1980), Arch. Biochem. Biophys., 203, 372-383.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.35 0.0054
-
 NADH
-
 Rattus norvegicus
1.1.1.35 0.0163
-
 (S)-3-hydroxyoctanoyl-CoA mitochondrial enzyme, oxidation of (S)-3-hydroxyoctanoyl-CoA Rattus norvegicus
1.1.1.35 0.0169
-
 acetoacetyl-CoA
-
 Rattus norvegicus
1.1.1.35 0.0286
-
 (S)-3-hydroxyhexanoyl-CoA mitochondrial enzyme, oxidation of (S)-3-hydroxyhexanoyl-CoA Rattus norvegicus
1.1.1.35 0.0585
-
 NAD+
-
 Rattus norvegicus
1.1.1.35 0.0699
-
 (S)-3-hydroxybutyryl-CoA mitochondrial enzyme, oxidation of (S)-3-hydroxybutyryl-CoA Rattus norvegicus
1.1.1.35 0.088
-
 (S)-3-hydroxydecanoyl-CoA mitochondrial enzyme, oxidation of (S)-3-hydroxydecanoyl-CoA Rattus norvegicus
1.1.1.35 0.435
-
 NADPH
-
 Rattus norvegicus
1.1.1.35 0.56
-
 acetoacetyl-pantetheine
-
 Rattus norvegicus
1.1.1.35 11
-
 NADP+
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.35 mitochondrion
-
 Rattus norvegicus 5739
-
1.1.1.35 peroxisome
-
 Rattus norvegicus 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.35 32000
-
 2 * 32000, mitochondrial enzyme, SDS-PAGE Rattus norvegicus
1.1.1.35 65000
-
 mitochondrial enzyme, gel filtration Rattus norvegicus
1.1.1.35 70000
-
 peroxisomal enzyme, gel filtration Rattus norvegicus
1.1.1.35 77000
-
 1 * 77000, peroxisomal enzyme, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.35 (S)-3-hydroxybutyryl-CoA + NAD+ Rattus norvegicus
-
 acetoacetyl-CoA + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.35 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.35 calcium phosphate gel, ammonium sulfate, CM-Sephadex, Blue Dextran-Sepharose 4B, calcium phosphate gel-cellulose, Blue Dextran-Sepharose 4B, mitochondrial enzyme Rattus norvegicus
1.1.1.35 phosphocellulose, ammonium sulfate, CM-cellulose, ammonium sulfate, peroxisomal enzyme Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.35 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.35 12.6
-
 peroxisomal enzyme Rattus norvegicus
1.1.1.35 565
-
 mitochondrial enzyme Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.1.1.35 -20°C, 120 mM potassium phosphate, pH 7.0, several months without loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.35 (S)-3-hydroxybutyryl-CoA + NAD+
-
 Rattus norvegicus acetoacetyl-CoA + NADH
-
 r
1.1.1.35 (S)-3-hydroxybutyryl-CoA + NADP+ low activity for mitochondrial enzyme Rattus norvegicus acetoacetyl-CoA + NADPH
-
 r
1.1.1.35 (S)-3-hydroxydecanoyl-CoA + NAD+
-
 Rattus norvegicus 3-ketodecanoyl-CoA + NADH
-
 r
1.1.1.35 (S)-3-hydroxyhexanoyl-CoA + NAD+
-
 Rattus norvegicus 3-oxohexanoyl-CoA + NADH + H+
-
 r
1.1.1.35 3-ketooctanoyl-CoA + NADH
-
 Rattus norvegicus (S)-3-hydroxyoctanoyl-CoA + NAD+
-
 ?
1.1.1.35 acetoacetyl-pantetheine + NADH
-
 Rattus norvegicus (S)-3-hydroxybutyryl-pantetheine
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.35 dimer 2 * 32000, mitochondrial enzyme, SDS-PAGE Rattus norvegicus
1.1.1.35 monomer 1 * 77000, peroxisomal enzyme, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.35 4.5
-
 mitochondrial enzyme, reduction of acetoacetyl-CoA Rattus norvegicus
1.1.1.35 6.2
-
 peroxisomal enzyme, reduction of acetoacetyl-CoA Rattus norvegicus
1.1.1.35 9.6
-
 mitochondrial enzyme, oxidation of (S)-3-hydroxybutyryl-CoA Rattus norvegicus
1.1.1.35 9.8
-
 peroxisomal enzyme, oxidation of (S)-3-hydroxybutyryl-CoA Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.35 NAD+
-
 Rattus norvegicus
1.1.1.35 NADP+
-
 Rattus norvegicus