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Literature summary extracted from

  • Kim, D.Y.; Bochar, D.A.; Stauffacher, C.V.; Rodwell, V.W.
    Engineering of Sulfolobus solfataricus HMG-CoA reductase to a form whose activity is regulated by phosphorylation and dephosphorylation (2000), Biochemistry, 39, 2269-2275.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.34 expression in Escherichia coli Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.34 L403R/G404R/A406S regulation of activity by phosphorylation Saccharolobus solfataricus
1.1.1.34 L403R/G404R/A406S engineering of an appropriately located phosphoacceptor serine and cAMP-dependent protein kinase recognition motif to create a phosphorylation site. Km values, Vmax, optimal pH and temperature of mutant are identical to wild-type. Exposure of mutant to ATP and cAMP-dependent protein kinase is accompanied by incorporation of phosphate and by a parallel decrease in catalytic activity. Subsequent treatment with a protein phosphatase releases enzyme-bound 32Pi and restores activity to pretreatment levels Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.34 0.00011
-
 CoASH pH 9.0, 50°C, wild-type Saccharolobus solfataricus
1.1.1.34 0.00015
-
 CoASH pH 9.0, 50°C, mutant L403R/G404R/A406S Saccharolobus solfataricus
1.1.1.34 0.0009
-
 NADP+ pH 9.0, 50°C, wild-type Saccharolobus solfataricus
1.1.1.34 0.0013
-
 NADP+ pH 9.0, 50°C, mutant L403R/G404R/A406S Saccharolobus solfataricus
1.1.1.34 0.0015
-
 (R)-mevalonate pH 9.0, 50°C, wild-type Saccharolobus solfataricus
1.1.1.34 0.0015
-
 (R)-mevalonate wild type enzyme, at pH 9.0 and 50°C Saccharolobus solfataricus
1.1.1.34 0.0017
-
 (R)-mevalonate pH 9.0, 50°C, mutnant L403R/G404R/A406S Saccharolobus solfataricus
1.1.1.34 0.0017
-
 (R)-mevalonate mutant enzyme L403R/G404R/A406S, at pH 9.0 and 50°C Saccharolobus solfataricus
1.1.1.34 0.0043
-
 mevaldehyde pH 5.0, 50°C, wild-type Saccharolobus solfataricus
1.1.1.34 0.0063
-
 mevaldehyde pH 5.0, 50°C, mutant L403R/G404R/A406S Saccharolobus solfataricus
1.1.1.34 0.045
-
 3-hydroxy-3-methylglutaryl-CoA wild type Saccharolobus solfataricus
1.1.1.34 0.045
-
 HMG-CoA pH 5.5, 50°C, wild-type Saccharolobus solfataricus
1.1.1.34 0.055
-
 NADPH wild type Saccharolobus solfataricus
1.1.1.34 0.055
-
 NADPH pH 5.5, 50°C, wild-type Saccharolobus solfataricus
1.1.1.34 0.076
-
 3-hydroxy-3-methylglutaryl-CoA mutant Saccharolobus solfataricus
1.1.1.34 0.076
-
 HMG-CoA pH 5.5, 50°C, mutant L403R/G404R/A406S Saccharolobus solfataricus
1.1.1.34 0.083
-
 NADPH mutant Saccharolobus solfataricus
1.1.1.34 0.083
-
 NADPH pH 5.5, 50°C, mutant L403R/G404R/A406S Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.34 3-hydroxy-3-methylglutaryl-CoA + NADPH Saccharolobus solfataricus
-
 (R)-mevalonate + CoA + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.34 Saccharolobus solfataricus
-
-
-
1.1.1.34 Saccharolobus solfataricus
-
 enzyme is not regulated by phosphorylation
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.34
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.34 3.9
-
 50°C, mutant L403R/G404R/A406S Saccharolobus solfataricus
1.1.1.34 4.9
-
 50°C, wild-type Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.34 (R)-mevalonate + CoA + 2 NADP+
-
 Saccharolobus solfataricus (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+
-
 r
1.1.1.34 (R)-mevalonate + CoA + NADP+
-
 Saccharolobus solfataricus (S)-3-hydroxy-3-methylglutaryl-CoA + NADPH + H+
-
 r
1.1.1.34 3-hydroxy-3-methylglutaryl-CoA + NADPH
-
 Saccharolobus solfataricus (R)-mevalonate + CoA + NADP+
-
 r
1.1.1.34 3-hydroxy-3-methylglutaryl-CoA + NADPH first step reaction Saccharolobus solfataricus (R)-mevalonate + CoA + NADP+
-
 r
1.1.1.34 mevaldehyde + NADPH + H+
-
 Saccharolobus solfataricus (R)-mevalonate + NADP+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.34 NADP+
-
 Saccharolobus solfataricus
1.1.1.34 NADPH
-
 Saccharolobus solfataricus