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Literature summary extracted from

  • Kim, D.Y.; Bochar, D.A.; Stauffacher, C.V.; Rodwell, V.W.
    Expression and characterization of the HMG-CoA reductase of the thermophilic archaeon Sulfolobus solfataricus (1999), Protein Expr. Purif., 17, 435-442.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.34 coexpression of the Sulfolobus solfataricus hmgA gene in Escherichia coli with the argU gene that encodes tRNAAGA,AGG results in an over 10fold increase in enzyme yield Saccharolobus solfataricus
1.1.1.34 expression in Escherichia coli Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.34 synthesis due to use of rare codon, expression of enzyme in Escherichia coli is poor. Coexpression of the S. solfataricus hmgA gene with the argU gene that encodes tRNAAGA,AGG resulted in an over 10-fold increase in enzyme yield Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.34 Mevinolin competitive with 3-hydroxy-3-methylglutaryl-CoA Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.34 3-hydroxy-3-methylglutaryl-CoA + NADPH Saccharolobus solfataricus
-
(R)-mevalonate + CoA + NADP+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.34 Saccharolobus solfataricus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.34
-
Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.34 4.9
-
pH 5.5, 50°C Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.34 3-hydroxy-3-methylglutaryl-CoA + NADPH
-
Saccharolobus solfataricus (R)-mevalonate + CoA + NADP+
-
?
1.1.1.34 3-hydroxy-3-methylglutaryl-CoA + NADPH
-
Saccharolobus solfataricus (R)-mevalonate + CoA + NADP+
-
r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.34 5.5
-
-
Saccharolobus solfataricus
1.1.1.34 9.5
-
oxidative acylation of (R,S)-mevaldehyde or mevalonate Saccharolobus solfataricus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.34 NADPH
-
Saccharolobus solfataricus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.34 0.000005
-
Mevinolin pH 5.5, 50°C Saccharolobus solfataricus