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Literature summary extracted from

  • Kawachi, T.; Rudney, H.
    Solubilization and purification of beta-hydroxy-beta-methylglutaryl coenzyme A reductase from rat liver (1970), Biochemistry, 9, 1700-1705.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.34 1,10-phenanthroline
-
 Rattus norvegicus
1.1.1.34 acetoacetyl-CoA
-
 Rattus norvegicus
1.1.1.34 acetyl-CoA
-
 Rattus norvegicus
1.1.1.34 CoASH
-
 Rattus norvegicus
1.1.1.34 deoxycholate
-
 Rattus norvegicus
1.1.1.34 digitonine 2% digitonin, 80% inhibition Rattus norvegicus
1.1.1.34 Fe2+
-
 Rattus norvegicus
1.1.1.34 Fe3+
-
 Rattus norvegicus
1.1.1.34 p-hydroxymercuribenzoate 0.01 mM inhibited 97% of reduction Rattus norvegicus
1.1.1.34 SDS
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.34 0.006
-
 3-hydroxy-3-methylglutaryl-CoA only one enantiomer Rattus norvegicus
1.1.1.34 0.012
-
 3-hydroxy-3-methylglutaryl-CoA
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.34 microsome
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.34 217000 226000 gel filtration, ultracentrifugation Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.34 3-hydroxy-3-methylglutaryl-CoA + NADPH Rattus norvegicus
-
 (R)-mevalonate + CoA + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.34 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.34
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.34 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.34 0.03
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.1.1.34 -10°C, crude microsomal form, 10-20% loss of activity per week Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.34 3-hydroxy-3-methylglutaryl-CoA + NADPH
-
 Rattus norvegicus (R)-mevalonate + CoA + NADP+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.34 7
-
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.34 NADPH
-
 Rattus norvegicus