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Literature summary extracted from

  • McIntyre, J.O.; Latruffe, N.; Brenner, S.C.; Fleischer, S.
    Comparison of 3-hydroxybutyrate dehydrogenase from bovine heart and rat liver mitochondria (1988), Arch. Biochem. Biophys., 262, 85-98.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.30 phosphatidylcholine
-
 Rattus norvegicus
1.1.1.30 phosphatidylcholine
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.30 0.24
-
 NAD+ liver 3-hydroxybutyrate dehydrogenase Rattus norvegicus
1.1.1.30 0.36
-
 NAD+ heart 3-hydroxybutyrate dehydrogenase Bos taurus
1.1.1.30 1.05
-
 (R)-3-hydroxybutanoate heart 3-hydroxybutyrate dehydrogenase Bos taurus
1.1.1.30 1.32
-
 (R)-3-hydroxybutanoate liver 3-hydroxybutyrate dehydrogenase Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.30 mitochondrion
-
 Rattus norvegicus 5739
-
1.1.1.30 mitochondrion
-
 Bos taurus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.30 27000
-
 alpha4, 4 * 27000, sedimentation equilibrium Rattus norvegicus
1.1.1.30 27000
-
 alpha4, 4 * 27000, sedimentation equilibrium Bos taurus
1.1.1.30 31000
-
 alpha4, 4 * 31000, SDS-PAGE Rattus norvegicus
1.1.1.30 31000
-
 alpha4, 4 * 31000, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.30 (R)-3-hydroxybutanoate + NAD+ Rattus norvegicus
-
 acetoacetate + NADH
-
 r
1.1.1.30 (R)-3-hydroxybutanoate + NAD+ Bos taurus
-
 acetoacetate + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.30 Bos taurus
-
-
-
1.1.1.30 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.30 phospholipase A2 treatment, controlled-pore glass beads Rattus norvegicus
1.1.1.30 phospholipase A2 treatment, controlled-pore glass beads Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.30 heart
-
 Rattus norvegicus
-
1.1.1.30 heart
-
 Bos taurus
-
1.1.1.30 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.30 91
-
 liver 3-hydroxybutyrate dehydrogenase, reconstituted with mitochondrial phospholipids Rattus norvegicus
1.1.1.30 145
-
 heart 3-hydroxybutyrate dehydrogenase, reconstituted with mitochondrial phospholipids Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.30 (R)-3-hydroxybutanoate + NAD+
-
 Rattus norvegicus acetoacetate + NADH
-
 r
1.1.1.30 (R)-3-hydroxybutanoate + NAD+
-
 Bos taurus acetoacetate + NADH
-
 r
1.1.1.30 (R)-3-hydroxybutanoate + NAD+
-
 Rattus norvegicus acetoacetate + NADH + H+
-
 r
1.1.1.30 (R)-3-hydroxybutanoate + NAD+
-
 Bos taurus acetoacetate + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.30 tetramer alpha4, 4 * 27000, sedimentation equilibrium Rattus norvegicus
1.1.1.30 tetramer alpha4, 4 * 27000, sedimentation equilibrium Bos taurus
1.1.1.30 tetramer alpha4, 4 * 31000, SDS-PAGE Rattus norvegicus
1.1.1.30 tetramer alpha4, 4 * 31000, SDS-PAGE Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.30 NAD+
-
 Rattus norvegicus
1.1.1.30 NAD+
-
 Bos taurus