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Literature summary extracted from

  • Izumi, Y.; Yoshida, T.; Kanzaki, H.; Toki, S.; Miyazaki, S.S.; Yamada, H.
    Purification and characterization of hydroxypyruvate reductase from a serine-producing methylotroph, Hyphomicrobium methylovorum GM2 (1990), Eur. J. Biochem., 190, 279-284.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.29 AgNO3
-
 Hyphomicrobium methylovorum
1.1.1.29 CuSO4 slight Hyphomicrobium methylovorum
1.1.1.29 Dihydroxyfumarate
-
 Hyphomicrobium methylovorum
1.1.1.29 HgCl2
-
 Hyphomicrobium methylovorum
1.1.1.29 additional information not inhibitory: citrate, NADP+, EDTA, 1,10-phenanthroline, iodoacetate, iodoacetamide, N-ethylmaleimide, hydroxylamine, semicarbazide, NaN3, NaF, KCN, L-ascorbate, dithiothreitol, pyridoxal 5'-phosphate Hyphomicrobium methylovorum
1.1.1.29 p-chloromercuribenzoate
-
 Hyphomicrobium methylovorum
1.1.1.29 phenylhydrazine
-
 Hyphomicrobium methylovorum
1.1.1.29 ZnCl2 slight Hyphomicrobium methylovorum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.29 0.055
-
 NADH
-
 Hyphomicrobium methylovorum
1.1.1.29 0.175
-
 Hydroxypyruvate
-
 Hyphomicrobium methylovorum
1.1.1.29 10.8
-
 glyoxylate
-
 Hyphomicrobium methylovorum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.29 38000
-
 2 * 38000, SDS-PAGE Hyphomicrobium methylovorum
1.1.1.29 69000 70000 gel filtration Hyphomicrobium methylovorum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.29 hydroxypyruvate + NADH Hyphomicrobium methylovorum
-
 D-glycerate + NAD+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.29 Hyphomicrobium methylovorum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.29
-
 Hyphomicrobium methylovorum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.29 1086
-
-
 Hyphomicrobium methylovorum

Storage Stability

EC Number Storage Stability Organism
1.1.1.29 -20°C, 10 mM potassium phosphate buffer, pH 7.0, 45% v/v glycerol, 1.0 M DTT, 18 months Hyphomicrobium methylovorum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.29 glyoxylate + NADH
-
 Hyphomicrobium methylovorum glycolate + NAD+
-
 ?
1.1.1.29 hydroxypyruvate + NADH
-
 Hyphomicrobium methylovorum D-glycerate + NAD+
-
 ir

Subunits

EC Number Subunits Comment Organism
1.1.1.29 dimer 2 * 38000, SDS-PAGE Hyphomicrobium methylovorum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.29 25
-
 up to Hyphomicrobium methylovorum
1.1.1.29 30
-
 30 min, 13.3% loss of activity Hyphomicrobium methylovorum
1.1.1.29 35
-
 30 min, 30.8% loss of activity Hyphomicrobium methylovorum
1.1.1.29 40
-
 30 min, 100% loss of activity Hyphomicrobium methylovorum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.29 6.8
-
-
 Hyphomicrobium methylovorum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.29 5 9
-
 Hyphomicrobium methylovorum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.29 NADH specific for, not replaceable by NADPH Hyphomicrobium methylovorum