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Literature summary extracted from

  • Bryant, F.O.
    Characterization of the fructose 1,6-bisphosphate-activated, L(+)-lactate dehydrogenase from Thermoanaerobacter ethanolicus (1991), J. Enzyme Inhib., 5, 235-248.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.27 fructose 1,6-diphosphate L-lactate dehydrogenase form which is activated by fructose 1,6-diphosphate Thermoanaerobacter ethanolicus
1.1.1.27 fructose 1,6-diphosphate at pH 7.5 lactate dehydrogenase activity is absent without fructose 1,6-diphosphate, from pH 5.0 to pH 7.0 lactate dehydrogenase is present without fructose 1,6-diphosphate but is greater in the presence of the activator Thermoanaerobacter ethanolicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.27 NADPH
-
 Thermoanaerobacter ethanolicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.27 0.08
-
 NADH
-
 Thermoanaerobacter ethanolicus
1.1.1.27 0.25
-
 pyruvate with 1.0 mM fructose 1,6-diphosphate Thermoanaerobacter ethanolicus
1.1.1.27 2.2
-
 pyruvate in absence of fructose 1,6-diphosphate Thermoanaerobacter ethanolicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.27 33500
-
 4 * 33500, SDS-PAGE Thermoanaerobacter ethanolicus
1.1.1.27 134000
-
 gel filtration Thermoanaerobacter ethanolicus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.27 Thermoanaerobacter ethanolicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.27
-
 Thermoanaerobacter ethanolicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.27 589
-
-
 Thermoanaerobacter ethanolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.27 2-oxobutyrate + NADH
-
 Thermoanaerobacter ethanolicus 2-hydroxybutyrate + NAD+
-
 ?
1.1.1.27 2-oxoglutarate + NADH + H+
-
 Thermoanaerobacter ethanolicus 2-hydroxyglutarate + NAD+
-
 ?
1.1.1.27 pyruvate + NADH + H+
-
 Thermoanaerobacter ethanolicus (S)-lactate + NAD+
-
 ?
1.1.1.27 pyruvate + NADPH + H+ 34% of the activity with NADH Thermoanaerobacter ethanolicus (S)-lactate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.27 tetramer 4 * 33500, SDS-PAGE Thermoanaerobacter ethanolicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.27 50
-
 900 min, stable Thermoanaerobacter ethanolicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.27 6.5
-
-
 Thermoanaerobacter ethanolicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.27 5 7 about 55% of maximal activity at pH 5.0 and at pH 7.0 Thermoanaerobacter ethanolicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.27 NADH coenzyme Thermoanaerobacter ethanolicus
1.1.1.27 NADPH coenzyme Thermoanaerobacter ethanolicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.27 0.375
-
 NADPH
-
 Thermoanaerobacter ethanolicus