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Literature summary extracted from

  • Mulkiewicz, E.; Zietara, M.S.; Stachowiak, K.; Skorkowski, E.F.
    Properties of lactate dehydrogenase from the isopod, Saduria entomon (2000), Comp. Biochem. Physiol. B, 126, 337-346.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.27 pyruvate at high concentrations Saduria entomon

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.27 0.08
-
 pyruvate at 0°C and at 5°C Saduria entomon
1.1.1.27 0.12
-
 pyruvate 10°C Saduria entomon
1.1.1.27 0.13
-
 pyruvate 15°C Saduria entomon
1.1.1.27 0.14
-
 pyruvate 20°C Saduria entomon
1.1.1.27 0.15
-
 pyruvate 25°C Saduria entomon
1.1.1.27 0.18
-
 pyruvate 30°C Saduria entomon
1.1.1.27 90.04
-
 (S)-lactate
-
 Saduria entomon

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.27 36000
-
 4 * 36000, SDS-PAGE Saduria entomon
1.1.1.27 140000
-
 gel filtration Saduria entomon

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.27 Saduria entomon
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.27
-
 Saduria entomon

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.27 pectoral muscle
-
 Saduria entomon
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.27 510
-
-
 Saduria entomon

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.27 (S)-lactate + NAD+
-
 Saduria entomon pyruvate + NADH + H+
-
 r
1.1.1.27 pyruvate + NADH + H+
-
 Saduria entomon (S)-lactate + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.27 tetramer 4 * 36000, SDS-PAGE Saduria entomon

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.27 65
-
 20 min, stable up to Saduria entomon
1.1.1.27 71.5
-
 20 min, 50% loss of activity Saduria entomon

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.27 6.5
-
 reduction of pyruvate Saduria entomon
1.1.1.27 9.1
-
 oxidation of lactate Saduria entomon

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.27 NAD+ coenzyme Saduria entomon
1.1.1.27 NADH coenzyme Saduria entomon