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Literature summary extracted from

  • Betsche, T.; Bosach, K.; Gerhardt, B.
    L-Lactate dehydrogenase from leaves of Capsella bursa-pastoris (L.) Med. I. Identification and partial characterization (1979), Planta, 146, 567-574.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.27 0.25
-
 pyruvate
-
 Capsella bursa-pastoris
1.1.1.27 13
-
 (S)-lactate
-
 Capsella bursa-pastoris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.27 cytosol
-
 Capsella bursa-pastoris 5829
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.27 Capsella bursa-pastoris
-
 L. Med
-
1.1.1.27 Cardamine hirsuta
-
-
-
1.1.1.27 Lactuca sativa
-
-
-
1.1.1.27 no activity in Rubus idaeus
-
 leaf
-
1.1.1.27 no activity in Taraxacum officinale
-
 leaf
-
1.1.1.27 no activity in Zea mays
-
 leaf
-
1.1.1.27 Raphanus sativus
-
-
-
1.1.1.27 Spinacia oleracea
-
-
-
1.1.1.27 Thlaspi arvense
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.27 partial Capsella bursa-pastoris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.27 leaf
-
 Spinacia oleracea
-
1.1.1.27 leaf
-
 Raphanus sativus
-
1.1.1.27 leaf
-
 Lactuca sativa
-
1.1.1.27 leaf
-
 Capsella bursa-pastoris
-
1.1.1.27 leaf
-
 Thlaspi arvense
-
1.1.1.27 leaf
-
 Cardamine hirsuta
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.27 (S)-lactate + NAD+
-
 Lactuca sativa pyruvate + NADH + H+
-
 r
1.1.1.27 (S)-lactate + NAD+
-
 Capsella bursa-pastoris pyruvate + NADH + H+
-
 r
1.1.1.27 pyruvate + NADH + H+
-
 Lactuca sativa (S)-lactate + NAD+
-
 r
1.1.1.27 pyruvate + NADH + H+
-
 Capsella bursa-pastoris (S)-lactate + NAD+
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.27 7
-
 reduction of pyruvate Capsella bursa-pastoris
1.1.1.27 9
-
 oxidation of lactate Capsella bursa-pastoris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.27 7.7 9.5 pH 7.7: about 60% of maximal activity, pH 9.5: about 30% of maximal activity, reduction of pyruvate Capsella bursa-pastoris

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.27 NAD+ coenzyme Lactuca sativa
1.1.1.27 NAD+ coenzyme Capsella bursa-pastoris
1.1.1.27 NADH coenzyme Lactuca sativa
1.1.1.27 NADH coenzyme Capsella bursa-pastoris