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Literature summary extracted from

  • Steinbuechel, A.; Schlegel, H.G.
    NAD-linked L(+)-lactate dehydrogenase from the strict aerobe Alcaligenes eutrophus. 1. Purification and properties (1983), Eur. J. Biochem., 130, 321-328.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.27 fructose 1,6-diphosphate not dependent on fructose 1,6-diphosphate Cupriavidus necator

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.27 3-Fluoropyruvate
-
Cupriavidus necator
1.1.1.27 Ag+
-
Cupriavidus necator
1.1.1.27 Cd2+ partial Cupriavidus necator
1.1.1.27 Cu2+
-
Cupriavidus necator
1.1.1.27 Hg2+
-
Cupriavidus necator
1.1.1.27 NAD+
-
Cupriavidus necator
1.1.1.27 Ni2+ partial Cupriavidus necator
1.1.1.27 oxalate
-
Cupriavidus necator
1.1.1.27 oxaloacetate
-
Cupriavidus necator
1.1.1.27 oxamate
-
Cupriavidus necator
1.1.1.27 p-chloromercuribenzoate
-
Cupriavidus necator
1.1.1.27 phosphoenolpyruvate
-
Cupriavidus necator
1.1.1.27 Zn2+
-
Cupriavidus necator

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.27 37000
-
2 * 37000, SDS-PAGE Cupriavidus necator
1.1.1.27 65000 67000 gel filtration Cupriavidus necator

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.27 Cupriavidus necator
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.27
-
Cupriavidus necator

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.27 824.6
-
-
Cupriavidus necator

Storage Stability

EC Number Storage Stability Organism
1.1.1.27 -20°C, stable for months Cupriavidus necator
1.1.1.27 stabilized for storage at 4°C by addition of 0.2-1 M potassium chloride or 3.2 M ammonium sulfate Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.27 (S)-lactate + NAD+
-
Cupriavidus necator pyruvate + NADH + H+
-
r
1.1.1.27 2-oxobutyrate + NADH 107% of the activity with pyruvate Cupriavidus necator 2-hydroxybutyrate + NAD+
-
?
1.1.1.27 2-oxoglutarate + NADH + H+ 79.6% of the activity with pyruvate Cupriavidus necator 2-hydroxyglutarate + NAD+
-
?
1.1.1.27 2-oxopentanoate + NADH 125% of the activity with pyruvate Cupriavidus necator 2-hydroxypentanoate + NAD+
-
?
1.1.1.27 3-fluoropyruvate + NADH 33.6% of the activity with pyruvate Cupriavidus necator ?
-
?
1.1.1.27 3-methyl-2-oxobutanoate + NADH 28.5% of the activity with pyruvate Cupriavidus necator 2-hydroxy-3-methylbutanoate + NAD+
-
?
1.1.1.27 3-methyl-2-oxopentanoate + NADH 5.3% of the activity with pyruvate Cupriavidus necator 2-hydroxy-3-methylpentanoate + NAD+
-
?
1.1.1.27 4-methyl-2-oxopentanoate + NADH 39% of the activity with pyruvate Cupriavidus necator 2-hydroxy-4-methylpentanoate + NAD+
-
?
1.1.1.27 pyruvate + NADH + H+
-
Cupriavidus necator (S)-lactate + NAD+
-
r

Subunits

EC Number Subunits Comment Organism
1.1.1.27 dimer 2 * 37000, SDS-PAGE Cupriavidus necator

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.27 42 45
-
Cupriavidus necator

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.27 7
-
reduction of pyruvate Cupriavidus necator
1.1.1.27 9.8
-
oxidation of lactate Cupriavidus necator

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.27 5.3 8 pH 5.3: about 50% of maximal activity, pH 8.0: about 60% of maximal activity, reduction of pyruvate Cupriavidus necator
1.1.1.27 8.5 10.7 pH 8.5: about 60% of maximal activity, pH 10.7: about 70% of maximal activity, oxidation of lactate Cupriavidus necator

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.27 NAD+ coenzyme Cupriavidus necator
1.1.1.27 NADH coenzyme Cupriavidus necator