EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.27 | fructose 1,6-diphosphate | not dependent on fructose 1,6-diphosphate | Cupriavidus necator |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.27 | 3-Fluoropyruvate | - |
Cupriavidus necator | |
1.1.1.27 | Ag+ | - |
Cupriavidus necator | |
1.1.1.27 | Cd2+ | partial | Cupriavidus necator | |
1.1.1.27 | Cu2+ | - |
Cupriavidus necator | |
1.1.1.27 | Hg2+ | - |
Cupriavidus necator | |
1.1.1.27 | NAD+ | - |
Cupriavidus necator | |
1.1.1.27 | Ni2+ | partial | Cupriavidus necator | |
1.1.1.27 | oxalate | - |
Cupriavidus necator | |
1.1.1.27 | oxaloacetate | - |
Cupriavidus necator | |
1.1.1.27 | oxamate | - |
Cupriavidus necator | |
1.1.1.27 | p-chloromercuribenzoate | - |
Cupriavidus necator | |
1.1.1.27 | phosphoenolpyruvate | - |
Cupriavidus necator | |
1.1.1.27 | Zn2+ | - |
Cupriavidus necator |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.27 | 37000 | - |
2 * 37000, SDS-PAGE | Cupriavidus necator |
1.1.1.27 | 65000 | 67000 | gel filtration | Cupriavidus necator |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.27 | Cupriavidus necator | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.27 | - |
Cupriavidus necator |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.27 | 824.6 | - |
- |
Cupriavidus necator |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.27 | -20°C, stable for months | Cupriavidus necator |
1.1.1.27 | stabilized for storage at 4°C by addition of 0.2-1 M potassium chloride or 3.2 M ammonium sulfate | Cupriavidus necator |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.27 | (S)-lactate + NAD+ | - |
Cupriavidus necator | pyruvate + NADH + H+ | - |
r | |
1.1.1.27 | 2-oxobutyrate + NADH | 107% of the activity with pyruvate | Cupriavidus necator | 2-hydroxybutyrate + NAD+ | - |
? | |
1.1.1.27 | 2-oxoglutarate + NADH + H+ | 79.6% of the activity with pyruvate | Cupriavidus necator | 2-hydroxyglutarate + NAD+ | - |
? | |
1.1.1.27 | 2-oxopentanoate + NADH | 125% of the activity with pyruvate | Cupriavidus necator | 2-hydroxypentanoate + NAD+ | - |
? | |
1.1.1.27 | 3-fluoropyruvate + NADH | 33.6% of the activity with pyruvate | Cupriavidus necator | ? | - |
? | |
1.1.1.27 | 3-methyl-2-oxobutanoate + NADH | 28.5% of the activity with pyruvate | Cupriavidus necator | 2-hydroxy-3-methylbutanoate + NAD+ | - |
? | |
1.1.1.27 | 3-methyl-2-oxopentanoate + NADH | 5.3% of the activity with pyruvate | Cupriavidus necator | 2-hydroxy-3-methylpentanoate + NAD+ | - |
? | |
1.1.1.27 | 4-methyl-2-oxopentanoate + NADH | 39% of the activity with pyruvate | Cupriavidus necator | 2-hydroxy-4-methylpentanoate + NAD+ | - |
? | |
1.1.1.27 | pyruvate + NADH + H+ | - |
Cupriavidus necator | (S)-lactate + NAD+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.27 | dimer | 2 * 37000, SDS-PAGE | Cupriavidus necator |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.27 | 42 | 45 | - |
Cupriavidus necator |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.27 | 7 | - |
reduction of pyruvate | Cupriavidus necator |
1.1.1.27 | 9.8 | - |
oxidation of lactate | Cupriavidus necator |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.27 | 5.3 | 8 | pH 5.3: about 50% of maximal activity, pH 8.0: about 60% of maximal activity, reduction of pyruvate | Cupriavidus necator |
1.1.1.27 | 8.5 | 10.7 | pH 8.5: about 60% of maximal activity, pH 10.7: about 70% of maximal activity, oxidation of lactate | Cupriavidus necator |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.27 | NAD+ | coenzyme | Cupriavidus necator | |
1.1.1.27 | NADH | coenzyme | Cupriavidus necator |