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Literature summary extracted from

  • Goetz, F.; Schleifer, K.H.
    Purification and properties of a fructose-1,6-diphosphate activated L-lactate dehydrogenase from Staphylococcus epidermidis (1975), Arch. Microbiol., 105, 303-312.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.27 fructose 1,6-diphosphate L-lactate dehydrogenase form which is activated by fructose 1,6-diphosphate Staphylococcus epidermidis
1.1.1.27 fructose 1,6-diphosphate concentration required for 50% maximal activity is about 0.15 mM Staphylococcus epidermidis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.27 ADP competitive with NADH Staphylococcus epidermidis
1.1.1.27 AMP
-
 Staphylococcus epidermidis
1.1.1.27 ATP
-
 Staphylococcus epidermidis
1.1.1.27 GTP
-
 Staphylococcus epidermidis
1.1.1.27 oxamate
-
 Staphylococcus epidermidis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.27 36000
-
 4 * 36000, SDS-PAGE Staphylococcus epidermidis
1.1.1.27 130000
-
 sucrose density gradient centrifugation Staphylococcus epidermidis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.27 Staphylococcus epidermidis
-
 ATCC 14990
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.27
-
 Staphylococcus epidermidis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.27 2220
-
-
 Staphylococcus epidermidis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.27 (S)-lactate + NAD+ poor reaction Staphylococcus epidermidis pyruvate + NADH + H+
-
 r
1.1.1.27 pyruvate + NADH + H+
-
 Staphylococcus epidermidis (S)-lactate + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.27 tetramer 4 * 36000, SDS-PAGE Staphylococcus epidermidis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.27 39
-
-
 Staphylococcus epidermidis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.27 50
-
 2 min, 6% loss of activity in presence of fructose 1,6-diphosphate, NADH and pyruvate, complete loss of activity without protectant Staphylococcus epidermidis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.27 5.6
-
 reduction of pyruvate Staphylococcus epidermidis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.27 5.2 7.2 pH 5.2: about 35% of maximal activity, pH 7.2: about 50% of maximal activity Staphylococcus epidermidis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.27 6.5
-
 highest stability at Staphylococcus epidermidis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.27 NAD+ coenzyme Staphylococcus epidermidis
1.1.1.27 NADH coenzyme Staphylococcus epidermidis