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Literature summary extracted from

  • Gordon, G.L.; Doelle, H.W.
    Purification, properties and immunological relationship of L (+)-lactate dehydrogenase from Lactobacillus casei (1976), Eur. J. Biochem., 67, 543-555.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.27 fructose 1,6-diphosphate L-lactate dehydrogenase form which is activated by fructose 1,6-diphosphate Lacticaseibacillus casei

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.27 6-phosphogluconate
-
 Lacticaseibacillus casei
1.1.1.27 ADP
-
 Lacticaseibacillus casei
1.1.1.27 ATP
-
 Lacticaseibacillus casei
1.1.1.27 citrate/phosphate buffer at pH 5.4 Lacticaseibacillus casei
1.1.1.27 Cu2+ CuSO4 Lacticaseibacillus casei
1.1.1.27 dihydroxyacetone phosphate
-
 Lacticaseibacillus casei
1.1.1.27 GTP
-
 Lacticaseibacillus casei
1.1.1.27 Hg2+ HgCl2 Lacticaseibacillus casei
1.1.1.27 NAD+
-
 Lacticaseibacillus casei
1.1.1.27 p-hydroxymercuribenzoate
-
 Lacticaseibacillus casei
1.1.1.27 Sodium phosphate
-
 Lacticaseibacillus casei
1.1.1.27 Tris/maleate buffer at pH 5.4 Lacticaseibacillus casei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.27 0.01
-
 NADH fructose 1,6-diphosphate has no effect Lacticaseibacillus casei
1.1.1.27 1
-
 pyruvate in presence of 0.02 mM fructose 1,6-diphosphate Lacticaseibacillus casei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.27 34000
-
 4 * 34000, SDS-PAGE Lacticaseibacillus casei
1.1.1.27 132000
-
 non-denaturing gel electrophoresis Lacticaseibacillus casei
1.1.1.27 135000
-
 gel filtration Lacticaseibacillus casei

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.27 Lacticaseibacillus casei
-
 ATCC 393
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.27 fructose 1,6-diphosphate-activated enzyme form Lacticaseibacillus casei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.27 2292
-
-
 Lacticaseibacillus casei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.27 pyruvate + NADH + H+
-
 Lacticaseibacillus casei (S)-lactate + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.27 tetramer 4 * 34000, SDS-PAGE Lacticaseibacillus casei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.27 70
-
 3 min, complete inactivation Lacticaseibacillus casei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.27 5.4
-
 sodium acetate buffer Lacticaseibacillus casei

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.27 NADH coenzyme Lacticaseibacillus casei