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Literature summary extracted from
- Characterization of 1-deoxy-D-xylulose 5-phosphate reductoisomerase, an enzyme involved in isopentenyl diphosphate biosynthesis, and identification of its catalytic amino acid residues (2000), J. Biol. Chem., 275, 19928-19932.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.267 | overexpression in Escherichia coli | Escherichia coli |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.1.1.267 | E231K | less than 0.24% wild type kcat | Escherichia coli |
| 1.1.1.267 | G14D | mutant | Escherichia coli |
| 1.1.1.267 | H153Q | increase of Km | Escherichia coli |
| 1.1.1.267 | H209Q | increase of Km | Escherichia coli |
| 1.1.1.267 | H257Q | increase of Km | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.267 | fosmidomycin | - |
Escherichia coli |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.267 | 0.007 | - |
NADPH | with 1 mM MnCl2 | Escherichia coli | |
| 1.1.1.267 | 0.009 | - |
NADPH | with 1 mM CoCl2 | Escherichia coli | |
| 1.1.1.267 | 0.02 | - |
NADPH | with 1 mM MgCl2 | Escherichia coli | |
| 1.1.1.267 | 0.06 | - |
1-deoxy-D-xylulose 5-phosphate | with 1 mM CoCl2 | Escherichia coli | |
| 1.1.1.267 | 0.099 | - |
1-deoxy-D-xylulose 5-phosphate | with 1 mM MgCl2 | Escherichia coli | |
| 1.1.1.267 | 0.25 | - |
1-deoxy-D-xylulose 5-phosphate | with 1 mM MnCl2 | Escherichia coli | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.267 | Co2+ | required for activity | Escherichia coli | |
| 1.1.1.267 | Mg2+ | required for activity | Escherichia coli | |
| 1.1.1.267 | Mn2+ | required for activity | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.267 | 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | Escherichia coli | - |
2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
ir | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.267 | Escherichia coli | P45568 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.267 | of recombinant protein | Escherichia coli |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.267 | 4°C, 100 mM Tris-HCl pH 7.5, 1 month | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.267 | 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | - |
Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
ir | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.267 | 50 | 60 | - |
Escherichia coli |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.267 | 40 | 60 | - |
Escherichia coli |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.267 | 50 | - |
not heat-stable above | Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.267 | 7 | 8.5 | - |
Escherichia coli |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.267 | NADP+ | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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