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Literature summary extracted from

  • Stoop, J.M.H.; Williamson, J.D.; Conkling, M.A.; MacKay, J.J.; Pharr, D.M.
    Characterization of NAD-dependent mannitol dehydrogenase from celery as affected by ions, chelators, reducing agents and metabolites (1998), Plant Sci., 131, 43-51.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.255 1,10-phenanthroline dose dependent decrease in enzyme activity Apium graveolens
1.1.1.255 1,4-dithioerythritol 1.09 mM, 50% inhibition, partially reversed by diamide Apium graveolens
1.1.1.255 1,4-dithiothreitol 0.57 mM, 50% inhibition, reversed by diamide Apium graveolens
1.1.1.255 2-mercaptoethanol 30 mM, 50% inhibition, partially reversed by diamide Apium graveolens
1.1.1.255 ADP Ki: 0.24 mM at pH 7.0 Apium graveolens
1.1.1.255 AMP Ki: 0.50-0.80 mM at pH 9.5, Ki = 0.64 mM at pH 7.0 Apium graveolens
1.1.1.255 ATP Ki: 1.10 mM at pH 7.0 Apium graveolens
1.1.1.255 additional information EDTA and EGTA did not affect enzyme activity Apium graveolens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.255 0.00127
-
 NADH
-
 Apium graveolens
1.1.1.255 0.14
-
 NAD+ at pH 9.5 Apium graveolens
1.1.1.255 0.23
-
 NAD+ at pH 7.0 Apium graveolens
1.1.1.255 64
-
 D-mannitol at pH 9.5 Apium graveolens
1.1.1.255 400
-
 D-mannitol at pH 7.0 Apium graveolens
1.1.1.255 745
-
 D-mannose
-
 Apium graveolens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.255 Apium graveolens
-
 L. var. dulce Mill. Pers.
-
1.1.1.255 Apium graveolens
-
 celery
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.255 1.9
-
 at pH 9.5 Apium graveolens
1.1.1.255 2.2
-
 at pH 7.0 Apium graveolens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.255 D-mannitol + NAD+
-
 Apium graveolens D-mannose + NADH + H+
-
 r
1.1.1.255 additional information no cinnamyl alcohol dehydrogenase activity Apium graveolens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.255 MTD
-
 Apium graveolens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.255 6.5
-
 D-mannose reduction Apium graveolens
1.1.1.255 9.5
-
 D-mannitol oxidation Apium graveolens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.255 NAD+
-
 Apium graveolens