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Literature summary extracted from

  • Stoop, J.M.H.; Chilton, W.S.; Pharr, D.M.
    Substrate stereospecificity of the NAD-dependent mannitol dehydrogenase from celery (1996), Phytochemistry, 43, 1145-1150.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.255 D-mannose competitive inhibition, Ki: 200 mM Apium graveolens
1.1.1.255 D-threose competitive inhibition, Ki: 18 mM Apium graveolens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.255 40000
-
 1 * 40000, SDS-PAGE Apium graveolens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.255 D-arabinitol + NAD+ Apium graveolens
-
 D-lyxose + NADH + H+
-
 r
1.1.1.255 D-iditol + NAD+ Apium graveolens
-
 D-idose + NADH
-
 r
1.1.1.255 D-mannitol + NAD+ Apium graveolens highest activity, aldoses having 2S chirality are not reducible, with the exception of D-idose, marked preference for aldopentose and aldohexose, same stereospecificity in either direction with the same absolute configuration at C-2 D-mannose + NADH + H+
-
 r
1.1.1.255 D-talose + NADH Apium graveolens
-
 D-altritol + NAD+
-
 ir
1.1.1.255 D-threitol + NAD+ Apium graveolens 25% activity D-threose + NADH
-
 r
1.1.1.255 i-erythreitol + NAD+ Apium graveolens 16% activity ? + NADH
-
 ?
1.1.1.255 i-ribitol + NAD+ Apium graveolens
-
 L-ribose + NADH
-
 r
1.1.1.255 L-arabinitol + NAD+ Apium graveolens
-
 L-arabinose + NADH
-
 r
1.1.1.255 L-iditol + NAD+ Apium graveolens less than 10% activity L-idose + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.255 Apium graveolens
-
 celery
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.255 cell culture
-
 Apium graveolens
-
1.1.1.255 culture condition:mannitol-grown cell
-
 Apium graveolens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.255 1.32
-
 D-mannitol Apium graveolens

Storage Stability

EC Number Storage Stability Organism
1.1.1.255 -80°C, stable for several months Apium graveolens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.255 D-arabinitol + NAD+
-
 Apium graveolens D-lyxose + NADH + H+
-
 r
1.1.1.255 D-glucitol + NAD+ not oxidized by higher plants Apium graveolens D-gulose + NADH
-
 r
1.1.1.255 D-iditol + NAD+
-
 Apium graveolens D-idose + NADH
-
 r
1.1.1.255 D-mannitol + NAD+ highest activity, aldoses having 2S chirality are not reducible, with the exception of D-idose, marked preference for aldopentose and aldohexose, same stereospecificity in either direction with the same absolute configuration at C-2 Apium graveolens D-mannose + NADH + H+
-
 r
1.1.1.255 D-talose + NADH
-
 Apium graveolens D-altritol + NAD+
-
 ir
1.1.1.255 D-threitol + NAD+ 25% activity Apium graveolens D-threose + NADH
-
 r
1.1.1.255 i-erythreitol + NAD+ 16% activity Apium graveolens ? + NADH
-
 ?
1.1.1.255 i-galactitol + NAD+ not oxidized by higher plants Apium graveolens L-galactose + NADH
-
 r
1.1.1.255 i-ribitol + NAD+
-
 Apium graveolens L-ribose + NADH
-
 r
1.1.1.255 L-arabinitol + NAD+
-
 Apium graveolens L-arabinose + NADH
-
 r
1.1.1.255 L-iditol + NAD+ less than 10% activity Apium graveolens L-idose + NADH
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.255 monomer 1 * 40000, SDS-PAGE Apium graveolens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.255 MTD
-
 Apium graveolens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.255 NAD+
-
 Apium graveolens