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Literature summary extracted from

  • Maclean, J.; Campbell, S.A.; Pollock, K.; Chackrewarthy, S.; Coggins, J.R.; Lapthorn, A.J.
    Crystallization and preliminary X-ray analysis of shikimate dehydrogenase from Escherichia coli (2000), Acta Crystallogr. Sect. D, 56, 512-515.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.25 gene aroE, overexpression in the aroE auxotrophic mutant strain AB2834 Escherichia coli
1.1.1.25 overexpression in Escherichia coli K12 Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.25
-
 Escherichia coli
1.1.1.25 purified recombinant enzyme, 20 mg/ml in 10 mM Tris-HCl, pH 7.6, 0.4 mM DTT, and 2.5 mM NADPH, 20°C, sitting-drop vapour-diffusion method, reservoir solution contains 1.65 M ammonium sulfate, 100 mM cacodylate buffer, pH 5.8, 1.5 ml of protein solution is mixed with an equal volume of reservoir solution, 10-12 days, cryoprotection with 20% v/v glycerol, X-ray diffraction structure determination and analysis at 2.3 A resolution Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.25 29400
-
 mass spectometry and calculation from DNA sequence Escherichia coli
1.1.1.25 29410
-
 mass spectrometry Escherichia coli
1.1.1.25 29414
-
 1 * 29414, mass spectrometry Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.25 shikimate + NADP+ Escherichia coli
-
 3-dehydroshikimate + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.25 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.25
-
 Escherichia coli
1.1.1.25 recombinant enzyme from aroE auxotrophic mutant strain AB2834 by ammonium sulfate fractionation, anion exchange chromatography, ultrafiltration, and gel filtration Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.1.1.25 -20°C, Tris-HCl, pH 7.5, 0.4 mM DTT, 50% glycerol, long-term Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.25 shikimate + NADP+
-
 Escherichia coli 3-dehydroshikimate + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.25 monomer 1 * 29400, mass spectrometry and gel filtration Escherichia coli
1.1.1.25 monomer 1 * 29414, mass spectrometry Escherichia coli
1.1.1.25 More structure analysis, dynamic light scattering measurements Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.25 NADP+
-
 Escherichia coli
1.1.1.25 NADPH
-
 Escherichia coli