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Literature summary extracted from

  • Arfman, N.; Van Beeumen, J.; De Vries, G.E.; Harder, W.; Dijkhuizen, L.
    Purification and characterization of an activator protein for methanol dehydrogenase from thermotolerant Bacillus sp. (1991), J. Biol. Chem., 266, 3955-3960.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.244 Activator protein activation in presence of Mg2+, in vitro: 3 mol soluble activator protein per mol enzyme, in vivo estimated as 1 mol per 17.5 mol enzyme, MW 50000 with two subunits of MW 27000 each, in presence of activator protein and Mg2+ enzyme possesses a high affinity active site for alcohol and NAD+, up to 40fold increase of activity, primarily of Vmax, slight decrease of Km-value for methanol, enzyme/activator-interaction is dilution-sensitive, no stimulation of formaldehyde reduction Bacillus methanolicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.244 additional information
-
 additional information biphasic kinetics with all alcoholic substrates and NAD+ in the presence of activator protein Bacillus methanolicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.244 cytoplasm
-
 Bacillus methanolicus 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.244 Mg2+ required, 1-2 mol per mol subunit, atomic absorption spectroscopy, activation by activator protein depends on Mg2+, Zn2+, Mn2+, Ca2+ cannot replace Mg2+ Bacillus methanolicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.244 methanol + NAD+ Bacillus methanolicus involved in initial methanol oxidation formaldehyde + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.244 Bacillus methanolicus
-
 thermotolerant methylotrophic strain C1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.244 purification of activator protein Bacillus methanolicus

Storage Stability

EC Number Storage Stability Organism
1.1.1.244 4-20°C, complete dissociation after 48 h Bacillus methanolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.244 methanol + NAD+ no activity with 2-propanol, 2,3-butandiol, mannitol and glycerol Bacillus methanolicus formaldehyde + NADH + H+
-
 ?
1.1.1.244 methanol + NAD+ involved in initial methanol oxidation Bacillus methanolicus formaldehyde + NADH + H+
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.244 57 59
-
 Bacillus methanolicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.244 50
-
 t1/2: above 2 min Bacillus methanolicus
1.1.1.244 60
-
 t1/2: 6 min Bacillus methanolicus
1.1.1.244 70
-
 t1/2: 2 min Bacillus methanolicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.244 9.5
-
 methanol oxidation Bacillus methanolicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.244 NAD+ required, specific for Bacillus methanolicus