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Literature summary extracted from

  • Vonck, J.; Arfman, N.; De Vries, G.E.; Van Beeumen, J.; Van Bruggen, E.F.J.; Dijkhuizen, L.
    Electron microscopic analysis and biochemical characterization of a novel methanol dehydrogenase from the thermotolerant Bacillus sp. C1 (1991), J. Biol. Chem., 266, 3949-3954.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.99.37 enzyme forms a decamer with 5fold symmetry, electron microscopy Bacillus sp. (in: Bacteria)

General Stability

EC Number General Stability Organism
1.1.1.244 dilution inactivates methanol dehydrogenase activity, not reductase activity, sucrose, 20% w/v, stabilizes formaldehyde reductase not methanol dehydrogenase activity, metal ions, such as Fe2+ and Zn2+, do not stabilize methanol dehydrogenase activity, glycerol does not stabilize methanol dehydrogenase activity, dithiothreitol stabilizes reductase, not methanol dehydrogenase activity, storage leads to dissociation Bacillus methanolicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.244 1,10-phenanthroline reduction of aldehyde Bacillus methanolicus
1.1.1.244 EDTA reduction of aldehyde Bacillus methanolicus
1.1.1.244 iodoacetate weak inhibition of aldehyde reduction Bacillus methanolicus
1.1.1.244 N-ethylmaleimide weak inhibition of aldehyde reduction Bacillus methanolicus
1.1.99.37 1,10-phenanthroline 85% inhibition at 1 mM Bacillus sp. (in: Bacteria)
1.1.99.37 EDTA 40% inhibition at 5 mM Bacillus sp. (in: Bacteria)
1.1.99.37 iodoacetate less than 10% inhibition at a concentration of 5 mM Bacillus sp. (in: Bacteria)
1.1.99.37 N-ethylmaleimide less than 10% inhibition at a concentration of 5 mM Bacillus sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.244 cytoplasm
-
 Bacillus methanolicus 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.244 Mg2+
-
 Bacillus methanolicus
1.1.1.244 Zn2+ required, 1 mol per mol subunit, atomic absorption spectroscopy Bacillus methanolicus
1.1.99.37 Zn2+ contains one zinc and two magnesium ions per subunit Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.37 43000
-
 10 * 43000, electron microscopy Bacillus sp. (in: Bacteria)
1.1.99.37 363000
-
 or above, sedimentation equilibrium centrifugation Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.244 methanol + NAD+ Bacillus methanolicus involved in initial methanol oxidation formaldehyde + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.244 Bacillus methanolicus
-
 thermotolerant methylotrophic strain C1
-
1.1.99.37 Bacillus sp. (in: Bacteria)
-
-
-
1.1.99.37 Bacillus sp. (in: Bacteria) C1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.244
-
 Bacillus methanolicus

Storage Stability

EC Number Storage Stability Organism
1.1.1.244 4-20°C, enzyme dissociates upon storage Bacillus methanolicus
1.1.99.37 -20°C, no dissociation occurrs when the isolated protein is stored frozen Bacillus sp. (in: Bacteria)
1.1.99.37 at room temperature or at 4 °C, it dissociats into smaller fragments, probably monomers or dimers Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.244 methanol + NAD+
-
 Bacillus methanolicus formaldehyde + NADH + H+
-
 ?
1.1.1.244 methanol + NAD+ involved in initial methanol oxidation Bacillus methanolicus formaldehyde + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.244 decamer decameric structure detected by electron microscopic analysis Bacillus methanolicus
1.1.99.37 decamer 10 * 43000, electron microscopy Bacillus sp. (in: Bacteria)

Synonyms

EC Number Synonyms Comment Organism
1.1.99.37 NAD-dependent methanol dehydrogenase
-
 Bacillus sp. (in: Bacteria)

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.244 NAD+ required, specific for Bacillus methanolicus
1.1.99.37 FAD
-
 Bacillus sp. (in: Bacteria)