Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Arfman, N.; Dijkhuizen, L.
    Methanol dehydrogenase from thermotolerant methylotroph Bacillus C1 (1990), Methods Enzymol., 188, 223-226.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.244 dilution inactivates methanol dehydrogenase activity, not reductase activity, sucrose, 20% w/v, stabilizes formaldehyde reductase not methanol dehydrogenase activity, metal ions, such as Fe2+ and Zn2+, do not stabilize methanol dehydrogenase activity, glycerol does not stabilize methanol dehydrogenase activity, dithiothreitol stabilizes reductase, not methanol dehydrogenase activity, storage leads to dissociation Bacillus methanolicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.244 cytoplasm
-
 Bacillus methanolicus 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.244 43000
-
 10 * 43000, SDS-PAGE Bacillus methanolicus
1.1.1.244 363000
-
 ultracentrifugation sedimentation equilibrium, dissociation occurs during the centrifugation time of 80 h Bacillus methanolicus
1.1.1.244 430000
-
 calculated from electron microscopic analysis and MW of subunit Bacillus methanolicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.244 methanol + NAD+ Bacillus methanolicus involved in initial methanol oxidation formaldehyde + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.244 Bacillus methanolicus
-
 thermotolerant methylotrophic strain C1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.244 Q-Sepharose, Phenyl-Superose, dehydrogenase activity is almost completely lost after purification, 34% reductase activity are recovered Bacillus methanolicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.244 19.6
-
 formaldehyde reductase activity Bacillus methanolicus

Storage Stability

EC Number Storage Stability Organism
1.1.1.244 -80 to 4°C, in the presence of DTT at least 4 days stable Bacillus methanolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.244 ethanol + NAD+ best substrate Bacillus methanolicus ethanal + NADH
-
 ?
1.1.1.244 methanol + NAD+ 44% of activity with ethanol, NADP+ cannot replace NAD+ Bacillus methanolicus formaldehyde + NADH + H+
-
 ?
1.1.1.244 methanol + NAD+ involved in initial methanol oxidation Bacillus methanolicus formaldehyde + NADH + H+
-
 r
1.1.1.244 n-butanol + NAD+ 87% of activity with ethanol Bacillus methanolicus n-butanal + NADH
-
 ?
1.1.1.244 n-propanol + NAD+ 71% of activity with ethanol Bacillus methanolicus n-propanal + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.244 decamer 10 * 43000, SDS-PAGE Bacillus methanolicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.244 50
-
-
 Bacillus methanolicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.244 6.7
-
 formaldehyde reduction Bacillus methanolicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.244 NAD+ required, specific for Bacillus methanolicus