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Literature summary extracted from

  • van der Werf, M.J.; Boot, A.M.
    Metabolism of carveol and dihydrocarveol in Rhodococcus erythropolis DCL14 (2000), Microbiology, 146, 1129-1141.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.3.99.25 dialysis results in a complete loss of carvone reductase activity Rhodococcus erythropolis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.99.25 additional information Rhodococcus erythropolis the enzyme participates in the carveol and dihydrocarveol degradation pathway of Rhodococcus erythropolis DCL14 ?
-
 ?
1.3.99.25 additional information Rhodococcus erythropolis DCL14 the enzyme participates in the carveol and dihydrocarveol degradation pathway of Rhodococcus erythropolis DCL14 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.243 Rhodococcus erythropolis
-
-
-
1.1.1.296 Rhodococcus erythropolis
-
 NAD+-dependent dihydrocarveol dehydrogenase activity is high in carveol- and dihydrocarveol grown cells
-
1.1.1.296 Rhodococcus erythropolis DCL14
-
 NAD+-dependent dihydrocarveol dehydrogenase activity is high in carveol- and dihydrocarveol grown cells
-
1.3.99.25 Rhodococcus erythropolis
-
 DCL14
-
1.3.99.25 Rhodococcus erythropolis DCL14
-
 DCL14
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.243 (-)-trans-carveol + NADP+ = (-)-carvone + NADPH + H+ steroselective conversion of (6S)-carveol stereomers from a diastereomeric mixture of (4R)- or (4S)-carveol Rhodococcus erythropolis
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.99.25 culture condition:monoterpene-grown cell
-
 Rhodococcus erythropolis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.243 0.002
-
 NADP+-dependent (4R)-carveol dehydrogenase, activity in extracts of cells grown on (4R)-limonene Rhodococcus erythropolis
1.1.1.243 0.002
-
 NADP+-dependent (4S)-carveol dehydrogenase, activity in extracts of cells grown on (4R)-carveol Rhodococcus erythropolis
1.1.1.243 0.005
-
 NADP+-dependent (4S)-carveol dehydrogenase, activity in extracts of cells grown on (4R)-limonene Rhodococcus erythropolis
1.1.1.243 0.005
-
 NADP+-dependent (4R)-carveol dehydrogenase, activity in extracts of cells grown on (4R)-carveol Rhodococcus erythropolis
1.1.1.243 0.009
-
 NAD+-dependent (4S)-carveol dehydrogenase, activity in extracts of cells grown on (4R)-limonene Rhodococcus erythropolis
1.1.1.243 0.014
-
 dichlorphenolindophenol+-dependent(4R)-carveol dehydrogenase, activity in extracts of cells grown on (4R)-carveol Rhodococcus erythropolis
1.1.1.243 0.014
-
 dichlorophenolindophenol-dependent (4S)-carveol dehydrogenase, activity in extracts of cells grown on (4R)-carveol Rhodococcus erythropolis
1.1.1.243 0.017
-
 NAD+-dependent (4R)-carveol dehydrogenase, activity in extracts of cells grown on (4R)-limonene Rhodococcus erythropolis
1.1.1.243 0.021
-
 NAD+-dependent(4S)-carveol dehydrogenase, activity in extracts of cells grown on (4R)-carveol Rhodococcus erythropolis
1.1.1.243 0.045
-
 NAD+-dependent (4R)-carveol dehydrogenase, activity in extracts of cells grown on (4R)-carveol Rhodococcus erythropolis
1.1.1.243 0.09
-
 dichlorophenolindophenol-dependent(4R)-carveol dehydrogenase, activity in extracts of cells grown on (4R)-limonene Rhodococcus erythropolis
1.1.1.243 0.105
-
 dichlorophenolindophenol+-dependent (4S)-carveol dehydrogenase, activity in extracts of cells grown on (4R)-limonene Rhodococcus erythropolis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.296 (1R,2R,4R)-dihydrocarveol + NAD+
-
 Rhodococcus erythropolis (1R,4R)-dihydrocarvone + NADH + H+
-
 ?
1.1.1.296 (1R,2R,4R)-dihydrocarveol + NAD+
-
 Rhodococcus erythropolis DCL14 (1R,4R)-dihydrocarvone + NADH + H+
-
 ?
1.1.1.296 (1R,2S,4R)-neo-dihydrocarveol + NAD+
-
 Rhodococcus erythropolis (1R,4R)-dihydrocarvone + NADH + H+
-
 ?
1.1.1.296 (1R,2S,4R)-neo-dihydrocarveol + NAD+
-
 Rhodococcus erythropolis DCL14 (1R,4R)-dihydrocarvone + NADH + H+
-
 ?
1.1.1.296 (1S,2R,4R)-neoisodihydrocarveol + NAD+
-
 Rhodococcus erythropolis (1S,4R)-iso-dihydrocarvone + NADH + H+
-
 ?
1.1.1.296 (1S,2R,4R)-neoisodihydrocarveol + NAD+
-
 Rhodococcus erythropolis DCL14 (1S,4R)-iso-dihydrocarvone + NADH + H+
-
 ?
1.1.1.296 (1S,2S,4R)-iso-dihydrocarveol + NAD+
-
 Rhodococcus erythropolis (1S,4R)-iso-dihydrocarvone + NADH + H+
-
 ?
1.1.1.296 (1S,2S,4R)-iso-dihydrocarveol + NAD+
-
 Rhodococcus erythropolis DCL14 (1S,4R)-iso-dihydrocarvone + NADH + H+
-
 ?
1.3.99.25 (4R)-carvone + reduced acceptor transient accumulation of (1R,4R)-dihydrocarvone is observed. Upon longer incubation, this compound is converted mainly into (1R,2R,4R)-dihydrocarveol and traces of (1R,2S,4R)-neo-dihydrocarveol Rhodococcus erythropolis ?
-
 ?
1.3.99.25 (4S)-carvone + reduced acceptor under aerobic conditions, only traces of (iso-)dihydrocarvone accumulate. Under anaerobic conditions much larger quantities of these compounds accumulate. The enzyme requires an unknown cofactor, which is different from NAD+, NADP+ or a flavin Rhodococcus erythropolis (1R,4S)-iso-dihydrocarvone + acceptor
-
 ?
1.3.99.25 additional information the enzyme participates in the carveol and dihydrocarveol degradation pathway of Rhodococcus erythropolis DCL14 Rhodococcus erythropolis ?
-
 ?
1.3.99.25 additional information the enzyme participates in the carveol and dihydrocarveol degradation pathway of Rhodococcus erythropolis DCL14 Rhodococcus erythropolis DCL14 ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.99.25 8
-
-
 Rhodococcus erythropolis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.243 NAD+
-
 Rhodococcus erythropolis
1.1.1.243 NADP+
-
 Rhodococcus erythropolis