Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Horiuchi, T.; Kurokawa, T.
    Purification and characterization of N-acetyl-D-hexosamine dehydrogenase from Pseudomonas sp. No. 53 (1989), Agric. Biol. Chem., 53, 1919-1925.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.1.1.240 stabilized against heating by 3 mM NAD+ Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.240 NaN3 weak Pseudomonas sp.
1.1.1.240 SDS
-
Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.240 0.24
-
NAD+ with N-acetyl-D-glucosamine as substrate Pseudomonas sp.
1.1.1.240 0.8
-
N-acetyl-D-galactosamine
-
Pseudomonas sp.
1.1.1.240 5.3
-
N-acetyl-D-glucosamine
-
Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.240 Hg2+ inhibitory Pseudomonas sp.
1.1.1.240 additional information not effected by Ca2+, Mg2+, Cd2+, Ni2+, Zn2+, Cu2+, Mn2+, Co2+ Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.240 30000
-
SDS-PAGE Pseudomonas sp.
1.1.1.240 124000
-
gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.240 N-acetyl-D-glucosamine + NAD+ Pseudomonas sp.
-
N-acetyl-D-glucosaminate + NADH
-
?
1.1.1.240 N-acetyl-D-glucosamine + NAD+ Pseudomonas sp. No. 53
-
N-acetyl-D-glucosaminate + NADH
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.240 Pseudomonas sp.
-
No. 53
-
1.1.1.240 Pseudomonas sp. No. 53
-
No. 53
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.240
-
Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.240 0.265
-
crude extract Pseudomonas sp.
1.1.1.240 239
-
purified enzyme Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.1.1.240 -20°C, several months, without much loss of activity Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.240 N-acetyl-D-galactosamine + NAD+
-
Pseudomonas sp. N-acetyl-D-galactoseaminate + NADH
-
?
1.1.1.240 N-acetyl-D-galactosamine + NAD+
-
Pseudomonas sp. No. 53 N-acetyl-D-galactoseaminate + NADH
-
?
1.1.1.240 N-acetyl-D-glucosamine + NAD+
-
Pseudomonas sp. N-acetyl-D-glucosaminate + NADH
-
?
1.1.1.240 N-acetyl-D-glucosamine + NAD+
-
Pseudomonas sp. No. 53 N-acetyl-D-glucosaminate + NADH
-
?
1.1.1.240 N-acetyl-D-mannosamine + NAD+ + H2O very poor substrate Pseudomonas sp. N-acetyl-D-mannosaminate + NADH + H+
-
?
1.1.1.240 N-acetyl-D-mannosamine + NAD+ + H2O very poor substrate Pseudomonas sp. No. 53 N-acetyl-D-mannosaminate + NADH + H+
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.240 tetramer 4 * 30000, SDS-PAGE, gel filtration Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.240 45
-
10 min, unstable below pH 5 and above pH 12 Pseudomonas sp.
1.1.1.240 55
-
10 min, stable Pseudomonas sp.
1.1.1.240 65
-
10 min, 50% loss of activity Pseudomonas sp.
1.1.1.240 70
-
10 min, complete loss of activity Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.240 655
-
N-acetyl-D-glucosamine
-
Pseudomonas sp.
1.1.1.240 932
-
N-acetyl-D-galactosamine
-
Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.240 10
-
-
Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.240 8 10.5 most stable Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.240 NAD+ strictly specific for NAD+ as hydrogen acceptor Pseudomonas sp.