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Literature summary extracted from
- Purification and characterization of quinate (shikimate) dehydrogenase, an enzyme in the inducible quinic acid catabolic pathway of Neurospora crassa (1978), Biochim. Biophys. Acta, 524, 1-14.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.24 | dithiothreitol | - |
Neurospora crassa |  |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.1.1.24 | high ionic strength, such as 0.1 M (NH4)2SO4, NaCl or phosphate buffer or the presence of 0.1 M quinate or shikimate protects against thermal inactivation | Neurospora crassa |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.24 | p-chloromercuribenzoate | - |
Neurospora crassa | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.24 | 0.32 | 0.37 | quinate | - |
Neurospora crassa | |
| 1.1.1.24 | 1.18 | - |
shikimate | - |
Neurospora crassa | |
| 1.1.1.282 | 0.37 | - |
L-quinate | - |
Neurospora crassa | |
| 1.1.1.282 | 1.18 | - |
shikimate | - |
Neurospora crassa | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.24 | 41000 | - |
1 * 41000, SDS-PAGE in presence of urea or electrophoresis in presence of cetyltrimethyl ammonium bromide | Neurospora crassa |
| 1.1.1.24 | 42000 | - |
sucrose density gradient centrifugation | Neurospora crassa |
| 1.1.1.282 | 41000 | - |
1 * 41000 | Neurospora crassa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.24 | quinate + NAD+ | Neurospora crassa | first reaction in inducible quinic acid catabolic pathway | 5-dehydroquinate + NADH + H+ | - |
? | |
| 1.1.1.282 | additional information | Neurospora crassa | catalyzes the first reaction in the inducible quinic acid catabolic pathway | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.24 | Neurospora crassa | - |
bifunctional enzyme: quinate (shikimate) dehydrogenase | - |
| 1.1.1.282 | Neurospora crassa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.24 | bifunctional enzyme: quinate (shikimate) dehydrogenase | Neurospora crassa |
| 1.1.1.282 | - |
Neurospora crassa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.24 | mycelium | - |
Neurospora crassa | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.24 | 86.87 | - |
- |
Neurospora crassa |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.24 | -20°C, 0.1 M NaCl, half-life 40 days | Neurospora crassa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.24 | quinate + NAD+ | - |
Neurospora crassa | 5-dehydroquinate + NADH + H+ | - |
? | |
| 1.1.1.24 | quinate + NAD+ | first reaction in inducible quinic acid catabolic pathway | Neurospora crassa | 5-dehydroquinate + NADH + H+ | - |
? | |
| 1.1.1.24 | shikimate + NAD+ | - |
Neurospora crassa | ? | - |
? | |
| 1.1.1.282 | L-quinate + NAD(P)+ | bifunctional enzyme with a single binding site for both substrates quinate and shikimate, the velocity is approximately 3fold lower with quinate than with shikimate | Neurospora crassa | 3-dehydroquinate + NAD(P)H + H+ | - |
? | |
| 1.1.1.282 | additional information | catalyzes the first reaction in the inducible quinic acid catabolic pathway | Neurospora crassa | ? | - |
? | |
| 1.1.1.282 | shikimate + NAD(P)+ | bifunctional enzyme with a single binding site for both substrates quinate and shikimate, the velocity is approximately 3fold higher with shikimate than with quinate | Neurospora crassa | 3-dehydroshikimate + NAD(P)H + H+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.24 | monomer | 1 * 41000, SDS-PAGE in presence of urea or electrophoresis in presence of cetyltrimethyl ammonium bromide | Neurospora crassa |
| 1.1.1.282 | monomer | 1 * 41000 | Neurospora crassa |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.24 | 40 | - |
half-life of unprotected enzyme: 20 min, 180 min in presence of NaCl, completely stable in presence of quinate, shikimate or NADH | Neurospora crassa |
| 1.1.1.282 | additional information | - |
the thermal stability of enzyme is greatly enhanced by low concentrations of quinate, shikimate, NADH or high ionic strength | Neurospora crassa |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.24 | 7.5 | - |
-20°C, highest stability | Neurospora crassa |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.24 | NAD+ | specific for NAD+ | Neurospora crassa | |
| 1.1.1.24 | NADH | specific for NAD+ | Neurospora crassa | |
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