BRENDA - Enzyme Database

Purification and properties of 3alpha-hydroxyglycyrrhetinate dehydrogenase of Clostridium innocuum from human intestine

Akao, T.; Akao, T.; Hattori, M.; Namba, T.; Kobashi, K.; J. Biochem. 103, 504-507 (1988)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.230
0.00027
-
NADP+
-
Clostridium innocuum
1.1.1.230
0.001
-
3-Ketoglycyrrhetic acid
-
Clostridium innocuum
1.1.1.230
0.002
-
3alpha-Hydroxyglycyrrhetic acid
-
Clostridium innocuum
1.1.1.230
0.024
-
NADPH
-
Clostridium innocuum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.230
soluble
-
Clostridium innocuum
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.230
30000
-
2 * 30000, SDS-PAGE
Clostridium innocuum
1.1.1.230
53000
-
gel filtration
Clostridium innocuum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.230
3alpha-hydroxyglycyrrhetic acid + NADP+
Clostridium innocuum
oxidation of 3alpha-hydroxy group faster than reduction of 3-keto group
3-ketoglycyrrhetic acid + NADPH
-
Clostridium innocuum
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.230
Clostridium innocuum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.230
-
Clostridium innocuum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.230
3alpha-hydroxy-18alpha-glycyrrhetic acid + NADP+
-
286341
Clostridium innocuum
3-keto-18alpha-glycyrrhetic acid + NADPH
-
286341
Clostridium innocuum
r
1.1.1.230
3alpha-hydroxyglycyrrhetic acid + NADP+
oxidation of 3alpha-hydroxy group faster than reduction of 3-keto group, not accepted as substrates: 3beta-hydroxyglycyrrhetic acid, steroids or bile acids containing 3alpha-hydroxy or 3-keto group
286341
Clostridium innocuum
3-ketoglycyrrhetic acid + NADPH
-
286341
Clostridium innocuum
r
1.1.1.230
3alpha-hydroxyglycyrrhetic acid + NADP+
oxidation of 3alpha-hydroxy group faster than reduction of 3-keto group
286341
Clostridium innocuum
3-ketoglycyrrhetic acid + NADPH
-
286341
Clostridium innocuum
r
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.230
dimer
2 * 30000, SDS-PAGE
Clostridium innocuum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.230
6
-
reduction of 3-keto group
Clostridium innocuum
1.1.1.230
8
-
oxidation of 3alpha-hydroxy group
Clostridium innocuum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.230
NADP+
-
Clostridium innocuum
1.1.1.230
NADPH
-
Clostridium innocuum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.230
NADP+
-
Clostridium innocuum
1.1.1.230
NADPH
-
Clostridium innocuum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.230
0.00027
-
NADP+
-
Clostridium innocuum
1.1.1.230
0.001
-
3-Ketoglycyrrhetic acid
-
Clostridium innocuum
1.1.1.230
0.002
-
3alpha-Hydroxyglycyrrhetic acid
-
Clostridium innocuum
1.1.1.230
0.024
-
NADPH
-
Clostridium innocuum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.230
soluble
-
Clostridium innocuum
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.230
30000
-
2 * 30000, SDS-PAGE
Clostridium innocuum
1.1.1.230
53000
-
gel filtration
Clostridium innocuum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.230
3alpha-hydroxyglycyrrhetic acid + NADP+
Clostridium innocuum
oxidation of 3alpha-hydroxy group faster than reduction of 3-keto group
3-ketoglycyrrhetic acid + NADPH
-
Clostridium innocuum
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.230
-
Clostridium innocuum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.230
3alpha-hydroxy-18alpha-glycyrrhetic acid + NADP+
-
286341
Clostridium innocuum
3-keto-18alpha-glycyrrhetic acid + NADPH
-
286341
Clostridium innocuum
r
1.1.1.230
3alpha-hydroxyglycyrrhetic acid + NADP+
oxidation of 3alpha-hydroxy group faster than reduction of 3-keto group, not accepted as substrates: 3beta-hydroxyglycyrrhetic acid, steroids or bile acids containing 3alpha-hydroxy or 3-keto group
286341
Clostridium innocuum
3-ketoglycyrrhetic acid + NADPH
-
286341
Clostridium innocuum
r
1.1.1.230
3alpha-hydroxyglycyrrhetic acid + NADP+
oxidation of 3alpha-hydroxy group faster than reduction of 3-keto group
286341
Clostridium innocuum
3-ketoglycyrrhetic acid + NADPH
-
286341
Clostridium innocuum
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.230
dimer
2 * 30000, SDS-PAGE
Clostridium innocuum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.230
6
-
reduction of 3-keto group
Clostridium innocuum
1.1.1.230
8
-
oxidation of 3alpha-hydroxy group
Clostridium innocuum