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Literature summary extracted from

  • Nagai, A.; Kheirolomoom, A.; Ohta, D.
    Site-directed mutagenesis shows that the conserved cysteine residues of histidinol dehydrogensae are not essential for catalysis (1993), J. Biochem., 114, 856-861.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.23 expressed in Spodoptera frugiperda, SF9 cells Brassica oleracea

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.23 C112A conserved Cys-112 is not involved in catalysis, Cys-112 corresponds to Cys-116 in Salmonella typhimurium Brassica oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.23 0.009
-
 L-histidinol
-
 Brassica oleracea
1.1.1.23 0.053
-
 NAD+
-
 Brassica oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.23 chloroplast enzyme contains chloroplast transit peptide sequence Brassica oleracea 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.23 L-histidinal + NAD+ + H2O Brassica oleracea reaction intermediate L-histidine + NADH + H+
-
 ?
1.1.1.23 L-histidinol + 2 NAD+ + H2O Brassica oleracea
-
 L-histidine + 2 NADH + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.23 Brassica oleracea
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.23 L-histidinal + NAD+ + H2O
-
 Brassica oleracea L-histidine + NADH + H+
-
 ?
1.1.1.23 L-histidinal + NAD+ + H2O reaction intermediate Brassica oleracea L-histidine + NADH + H+
-
 ?
1.1.1.23 L-histidinol + 2 NAD+ + H2O
-
 Brassica oleracea L-histidine + 2 NADH + 2 H+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.23 35
-
 L-histidinol recombinant enzyme without transit peptide Brassica oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.23 NAD+
-
 Brassica oleracea