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Literature summary extracted from

  • Milstien, S.; Kaufman, S.
    The biosynthesis of tetrahydrobiopterin in rat brain. Purification and characterization of 6-pyruvoyl tetrahydropterin (2-oxo)reductase (1989), J. Biol. Chem., 264, 8066-8073.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.220 0.001
-
 6-pyruvoyltetrahydropterin
-
 Rattus norvegicus
1.1.1.220 0.1
-
 4-nitrobenzaldehyde
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.220 37000
-
 SDS-PAGE, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.220 6-pyruvoyltetrahydropterin + NADPH Rattus norvegicus involved in de novo biosynthesis of tetrahydrobiopterin 6-lactoyl-5,6,7,8-tetrahydropterin + NADP+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.220 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.220 from brain Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.220 brain highest concentration in striatum and midbrain Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.220 22
-
 crude extract Rattus norvegicus
1.1.1.220 49300
-
 purified enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.220 4-nitrobenzaldehyde + NADPH
-
 Rattus norvegicus 4-nitrobenzylalcohol + NADP+
-
 ?
1.1.1.220 6-pyruvoyltetrahydropterin + NADPH
-
 Rattus norvegicus 6-lactoyl-5,6,7,8-tetrahydropterin + NADP+
-
 ir
1.1.1.220 6-pyruvoyltetrahydropterin + NADPH involved in de novo biosynthesis of tetrahydrobiopterin Rattus norvegicus 6-lactoyl-5,6,7,8-tetrahydropterin + NADP+
-
 ir
1.1.1.220 menadione + NADPH
-
 Rattus norvegicus ?
-
 ?
1.1.1.220 Phenanthrenequinone + NADPH
-
 Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.220 monomer SDS-PAGE, gel filtration Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.220 NADPH
-
 Rattus norvegicus