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Literature summary extracted from
- The biosynthesis of tetrahydrobiopterin in rat brain. Purification and characterization of 6-pyruvoyl tetrahydropterin (2-oxo)reductase (1989), J. Biol. Chem., 264, 8066-8073.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.220 | 0.001 | - |
6-pyruvoyltetrahydropterin | - |
Rattus norvegicus |  |
| 1.1.1.220 | 0.1 | - |
4-nitrobenzaldehyde | - |
Rattus norvegicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.220 | 37000 | - |
SDS-PAGE, gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.220 | 6-pyruvoyltetrahydropterin + NADPH | Rattus norvegicus | involved in de novo biosynthesis of tetrahydrobiopterin | 6-lactoyl-5,6,7,8-tetrahydropterin + NADP+ | - |
ir | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.220 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.220 | from brain | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.220 | brain | highest concentration in striatum and midbrain | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.220 | 22 | - |
crude extract | Rattus norvegicus |
| 1.1.1.220 | 49300 | - |
purified enzyme | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.220 | 4-nitrobenzaldehyde + NADPH | - |
Rattus norvegicus | 4-nitrobenzylalcohol + NADP+ | - |
? | |
| 1.1.1.220 | 6-pyruvoyltetrahydropterin + NADPH | - |
Rattus norvegicus | 6-lactoyl-5,6,7,8-tetrahydropterin + NADP+ | - |
ir | |
| 1.1.1.220 | 6-pyruvoyltetrahydropterin + NADPH | involved in de novo biosynthesis of tetrahydrobiopterin | Rattus norvegicus | 6-lactoyl-5,6,7,8-tetrahydropterin + NADP+ | - |
ir | |
| 1.1.1.220 | menadione + NADPH | - |
Rattus norvegicus | ? | - |
? | |
| 1.1.1.220 | Phenanthrenequinone + NADPH | - |
Rattus norvegicus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.220 | monomer | SDS-PAGE, gel filtration | Rattus norvegicus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.220 | NADPH | - |
Rattus norvegicus | |
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