Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Walker, E.H.; French, C.E.; Rathbone, D.A.; Bruce, N.C.
    Mechanistic studies of morphine dehydrogenase and stabilization against covalent inactivation (2000), Biochem. J., 345, 687-692.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.218 gene encoding morphine dehydrogenase on natural plasmid, expressed in Escherichia coli Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.218 C80S change of cysteine to serine: significantly improved stability Pseudomonas putida
1.1.1.218 D47N residue modified by site-directed mutagenesis, resulting in substantial loss of activity Pseudomonas putida
1.1.1.218 K76M residue modified by site-directed mutagenesis, resulting in substantial loss of activity Pseudomonas putida
1.1.1.218 Y52F residue modified by site-directed mutagenesis, resulting in substantial loss of activity Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.218 additional information competitive inhibition: NADP+ with respect to NADPH, codeine with respect to codeinone Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.218 0.12
-
 codeinone cosubstrate NADPH Pseudomonas putida
1.1.1.218 0.45
-
 codeine cosubstrate NADP+ Pseudomonas putida
1.1.1.218 0.54
-
 morphine cosubstrate NADP+ Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.218 31990
-
 based on sequence data Pseudomonas putida
1.1.1.218 32000
-
 elecrospray mass spectrometer Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.218 Pseudomonas putida
-
 M10
-
1.1.1.218 Pseudomonas putida M10
-
 M10
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.218 codeine + NAD(P)+ non-Theorell-Chance sequential ordered mechanism for codeine oxidation at pH 9.5 Pseudomonas putida codeinone + NAD(P)H morphine dehydrogenase follows a Theorell-Chance mechanism for codeinone reduction at pH 7.0 ?
1.1.1.218 codeine + NAD(P)+ non-Theorell-Chance sequential ordered mechanism for codeine oxidation at pH 9.5 Pseudomonas putida M10 codeinone + NAD(P)H morphine dehydrogenase follows a Theorell-Chance mechanism for codeinone reduction at pH 7.0 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.218 NAD(P)H enzyme specific for the proR hydrogen of NADPH Pseudomonas putida