EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.218 | Ketamine | - |
Mesocricetus auratus | |
1.1.1.218 | lithocholic acid | moderate inhibitory effect | Mesocricetus auratus | |
1.1.1.218 | additional information | competitive inhibitors: testosterone, 5alpha-androstan-17beta-ol-3-one, 5beta-androsteran-17beta-ol-3-one; no inhibition by indomethacine; no or weak inhibition by pyrazole, barbital | Mesocricetus auratus | |
1.1.1.218 | NEM | - |
Mesocricetus auratus | |
1.1.1.218 | p-chloromercuribenzoic acid | - |
Mesocricetus auratus | |
1.1.1.218 | Phenylarsine oxide | - |
Mesocricetus auratus | |
1.1.1.218 | quercetin | weak inhibitory effect | Mesocricetus auratus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.218 | 0.002 | - |
5alpha-androstan-17beta-ol-3-one | similar values of other 17beta-hydroxysteroids | Mesocricetus auratus | |
1.1.1.218 | 0.009 | - |
testosterone | cosubstrate NAD+ | Mesocricetus auratus | |
1.1.1.218 | 1.25 | - |
morphine | cosubstrate NAD+ | Mesocricetus auratus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.218 | cytosol | - |
Mesocricetus auratus | 5829 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.218 | 32000 | - |
native enzyme, gel filtration | Mesocricetus auratus |
1.1.1.218 | 38000 | - |
denatured enzyme, SDS-PAGE | Mesocricetus auratus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.218 | Mesocricetus auratus | - |
Syrian hamster | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.218 | gel filtration, various chromatographic techniques | Mesocricetus auratus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.218 | liver | - |
Mesocricetus auratus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.218 | 0.21 | - |
morphine, NAD+ | Mesocricetus auratus |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.218 | -80°C, 20% glycerol, v/v, stable at least four months | Mesocricetus auratus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.218 | codeine + NAD(P)+ | only weak enzyme activity for codeine | Mesocricetus auratus | codeinone + NAD(P)H | - |
? | |
1.1.1.218 | morphine + NADP+ | enzyme activity only with NAD+ | Mesocricetus auratus | morphinone + NADPH + H+ | - |
? | |
1.1.1.218 | normorphine + NAD(P)+ | best substrate, oxidation at 139% the rate of morphine oxidation | Mesocricetus auratus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.218 | monomer | 1 * 38000, SDS-PAGE | Mesocricetus auratus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.218 | 25 | - |
enzyme assay | Mesocricetus auratus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.218 | 9.3 | - |
in glycine-NaCl-NaOH buffer | Mesocricetus auratus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.218 | NAD+ | with NADP+ enzyme activity less than 5% those found with NAD+ | Mesocricetus auratus |