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Literature summary extracted from

  • Bruce, N.C.; Wilmot, C.J.; Jordan, K.N.; Stephens, L.D.G.; Lowe, Ch.R.
    Microbial degradation of the morphine alkaloids (1991), Biochem. J., 274, 875-880.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.1.1.218 2-mercaptoethanol stabilizes Pseudomonas putida
1.1.1.218 dithiothreitol stabilizes Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.218 1,10-phenanthroline
-
 Pseudomonas putida
1.1.1.218 2,2'-dipyridyl
-
 Pseudomonas putida
1.1.1.218 CuSO4
-
 Pseudomonas putida
1.1.1.218 iodoacetate
-
 Pseudomonas putida
1.1.1.218 additional information no inhibition by EDTA, NEM, hydroxyquinoline Pseudomonas putida
1.1.1.218 NEM inhibit activity at much higher concentration (e.g. at 1 mM) Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.218 0.044
-
 codeine with NADP+ Pseudomonas putida
1.1.1.218 0.35
-
 NADP+
-
 Pseudomonas putida
1.1.1.218 0.46
-
 morphine cosubstrate NADP+ Pseudomonas putida
1.1.1.218 2.91
-
 Dihydrocodeine cosubstrate NADP+ Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.218 31000
-
 denatured enzyme, SDS-PAGE Pseudomonas putida
1.1.1.218 32000
-
 native enzyme, gel filtration Pseudomonas putida
1.1.1.218 310000
-
 1 * 310000, SDS-PAGE Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.218 morphine + NADP+ Pseudomonas putida highly specific alkaloid dehydrogenase, oxidizing only the C-6 hydroxy group of morphine and codeine, morphine as the sole carbon and energy source morphinone + NADPH
-
 ?
1.1.1.218 morphine + NADP+ Pseudomonas putida M10 highly specific alkaloid dehydrogenase, oxidizing only the C-6 hydroxy group of morphine and codeine, morphine as the sole carbon and energy source morphinone + NADPH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.218 Pseudomonas putida
-
 M10
-
1.1.1.218 Pseudomonas putida M10
-
 M10
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.218 gel filtration, various chromatographic techniques Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.218 0.08
-
 morphine, NADP+ Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.1.1.218 -80°C, 10% loss of activity over 2 months Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.218 codeine + NAD(P)+ oxidation at 120% the rate of morphine oxidation Pseudomonas putida codeinone + NAD(P)H at pH 6.5, + NADPH, reduction of codeinone to codeine r
1.1.1.218 codeine + NAD(P)+ oxidation at 120% the rate of morphine oxidation Pseudomonas putida M10 codeinone + NAD(P)H at pH 6.5, + NADPH, reduction of codeinone to codeine r
1.1.1.218 dihydrocodeine + NADP+
-
 Pseudomonas putida dihydrocodeinone + NADPH
-
 ?
1.1.1.218 dihydrocodeine + NADP+
-
 Pseudomonas putida M10 dihydrocodeinone + NADPH
-
 ?
1.1.1.218 morphine + NADP+ enzyme activity only with NADP+ Pseudomonas putida morphinone + NADPH + H+
-
 ?
1.1.1.218 morphine + NADP+ enzyme activity only with NADP+ Pseudomonas putida M10 morphinone + NADPH + H+
-
 ?
1.1.1.218 morphine + NADP+ highly specific alkaloid dehydrogenase, oxidizing only the C-6 hydroxy group of morphine and codeine, morphine as the sole carbon and energy source Pseudomonas putida morphinone + NADPH
-
 ?
1.1.1.218 morphine + NADP+ highly specific alkaloid dehydrogenase, oxidizing only the C-6 hydroxy group of morphine and codeine, morphine as the sole carbon and energy source Pseudomonas putida M10 morphinone + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.218 monomer 1 * 310000, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.218 30
-
 enzyme assay Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.218 6.5
-
 in presence of NADPH reduction of codeinone to codeine, in MOPS buffer Pseudomonas putida
1.1.1.218 9.5
-
 with NADP+ as cofactor, in glycine-NaOH buffer, oxidizing the C-6 hydroxy group of morphine and codeine Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.218 NADP+ the Pseudomonas enzyme acts only with NADP+ Pseudomonas putida