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Literature summary extracted from

  • Yamano, S.; Nishida, F; Toki, S.
    Guinea-pig liver morphine 6-dehydrogenase as a naloxone reductase (1986), Biochem. Pharmacol., 35, 4321-4326.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.218 CuSO4
-
 Cavia porcellus
1.1.1.218 Ketamine
-
 Cavia porcellus
1.1.1.218 lithocholic acid
-
 Cavia porcellus
1.1.1.218 additional information no or weak inhibition by pyrazole, barbital Cavia porcellus
1.1.1.218 Phenylarsine oxide
-
 Cavia porcellus
1.1.1.218 prostaglandin E1
-
 Cavia porcellus
1.1.1.218 quercitrin
-
 Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.218 0.0022
-
 NADH
-
 Cavia porcellus
1.1.1.218 0.0065
-
 NADPH
-
 Cavia porcellus
1.1.1.218 0.27
-
 naloxone cosubstrate NADPH Cavia porcellus
1.1.1.218 0.44
-
 naloxone cosubstrate NADH Cavia porcellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.218 cytosol
-
 Cavia porcellus 5829
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.218 Cavia porcellus
-
-
-
1.1.1.218 Cavia porcellus Hartley
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.218 gel filtration, various chromatographic techniques Cavia porcellus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.218 liver
-
 Cavia porcellus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.218 0.86
-
 morphine, NADP+ Cavia porcellus
1.1.1.218 0.9
-
 naloxone, NADPH Cavia porcellus

Storage Stability

EC Number Storage Stability Organism
1.1.1.218 2-4°C, 50 mM sodium phosphate buffer + 100 mM 2-mercaptoethanol, pH 6., 90% of the activity retained with both NAD+ and NADP+ for a week Cavia porcellus
1.1.1.218 4°C, 80% of the activity after a week in 50 mM sodium phosphate buffer, pH 6.0, 100 mM 2-mercaptoethanol, more stable at pH 6.0 than at pH 8.0 Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.218 morphine + NADP+
-
 Cavia porcellus morphinone + NADPH + H+
-
 ?
1.1.1.218 morphine + NADP+
-
 Cavia porcellus Hartley morphinone + NADPH + H+
-
 ?
1.1.1.218 naloxone + NAD(P)H
-
 Cavia porcellus 6alpha-naloxol + NADP+ stereospecific reduction ?
1.1.1.218 naloxone + NAD(P)H
-
 Cavia porcellus Hartley 6alpha-naloxol + NADP+ stereospecific reduction ?
1.1.1.218 naloxone + NADH + H+
-
 Cavia porcellus ?
-
 r
1.1.1.218 naloxone + NADH + H+
-
 Cavia porcellus Hartley ?
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.218 naloxone reductase
-
 Cavia porcellus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.218 37
-
 enzyme assay, reduction of naloxone Cavia porcellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.218 6.2
-
 with NADH als cofactor, 67 mM sodium phosphate buffer Cavia porcellus
1.1.1.218 6.8
-
 with NADPH as cofactor, 67 mM sodium phosphate buffer Cavia porcellus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.218 6
-
 at pH6.0 more stable than at pH 8.0 on storage at 4°C Cavia porcellus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.218 NAD(P)+ utilized both with nearly equal efficiency Cavia porcellus
1.1.1.218 NAD(P)H NADPH: 2.7 times as effective as NADH Cavia porcellus