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Literature summary extracted from

  • Halder, A.B.; Crabbe, M.J.C.
    Bovine lens aldehyde reductase (aldose reductase) (1984), Biochem. J., 219, 33-39.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.21 2-[5-fluoro-2-methyl-1-p-(methylsulphinyl)benzylidine]indene-3-acetic acid non-competitive Bos taurus
1.1.1.21 disodium 4,6-dioxo-10-propyl-4H,6H-pyranol[3,2-g-]-quinoline-2,8-dicarboxylate non-competitive Bos taurus
1.1.1.21 glyceraldehyde substrate inhibition Bos taurus
1.1.1.21 malondialdehyde at concentration above 0.8 mM substrate inhibition Bos taurus
1.1.1.21 NADP+ competitve to NADPH Bos taurus
1.1.1.21 p-nitrobenzaldehyde at concentration above 2 mM substrate inhibition Bos taurus
1.1.1.21 sorbitol competitive inhibition with NADP+ and glucose Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.21 8.2
-
 NADPH
-
 Bos taurus
1.1.1.21 21.8
-
 glucose
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.21 32000
-
 gel filtration Bos taurus
1.1.1.21 40000
-
 1 * 40000, about, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.21 aldose + NAD(P)H Bos taurus
-
 alditol + NAD(P)+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.21 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.21
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.21 lens
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.21 0.2
-
 purified enzyme Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.21 alditol + NAD(P)+
-
 Bos taurus aldose + NAD(P)H
-
 r
1.1.1.21 aldose + NAD(P)H
-
 Bos taurus alditol + NAD(P)+
-
 r
1.1.1.21 D-fructose + NAD(P)H + H+
-
 Bos taurus D-arabino-hexitol + NAD(P)+
-
 r
1.1.1.21 D-glucose + NAD(P)H
-
 Bos taurus sorbitol + NAD(P)+
-
 ?
1.1.1.21 D-xylose + NAD(P)H
-
 Bos taurus xylitol + NAD(P)+
-
 r
1.1.1.21 DL-glyceraldehyde + NAD(P)H
-
 Bos taurus glycerol + NAD(P)+
-
 r
1.1.1.21 xylitol + NAD(P)+
-
 Bos taurus D-xylose + NAD(P)H + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.21 monomer 1 * 40000, about, SDS-PAGE Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.21 additional information
-
 inactive below pH 5.9 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.21 NADH
-
 Bos taurus
1.1.1.21 NADP+
-
 Bos taurus